Cytokine Factors Present in Dengue Patient Sera Induces Alterations of Junctional Proteins in Human Endothelial Cells
Cytokine Factors Present in Dengue Patient Sera Induces Alterations of Junctional Proteins in Human Endothelial Cells
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DOI：
10.4269/ajtmh.2012.11-0606
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发表时间：
2012-11-01
影响因子：
3.3
通讯作者：
Sekaran, Shamala Devi
中科院分区：
文献类型：
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作者：
Appanna, Ramapraba;Wang, Seok Mui;Sekaran, Shamala Devi
严重登革热中的血浆渗漏已被假定与偏斜的细胞因子免疫应答相关。在这项研究中，相关的细胞因子与血管通透性登革热患者进行了调查。采用Luminex Multiplex技术对从48名患者（13名登革热患者、29名登革出血热患者和6名健康人）采集的人血清样品进行细胞因子分析。然后测试来自登革出血热患者血清和重组人细胞因子的选定血清样品通过处理人脐静脉内皮细胞诱导血管通透性的作用。共聚焦免疫荧光染色显示，与健康人血清样品相比，用登革出血热患者血清样品处理的人脐静脉内皮细胞的形态学改变。这些发现表明，登革出血性感染过程中产生的细胞因子可以诱导血管内皮细胞的改变，这可能在登革的病理生理学中发挥重要作用。
Plasma leakage in severe dengue has been postulated to be associated with skewed cytokine immune responses. In this study, the association of cytokines with vascular permeability in dengue patients was investigated. Human serum samples collected from 48 persons (13 with dengue fever, 29 with dengue hemorrhagic fever, and 6 healthy) were subjected to cytokines analysis by using Luminex Multiplex Technology. Selected serum samples from patients with dengue hemorrhagic fever sera and recombinant human cytokines were then tested for roles on inducing vascular permeability by treatment of human umbilical vein endothelial cells. Confocal immunofluorescence staining indicated morphologic alteration of human umbilical vein endothelial cells treated with serum samples from patients with dengue hemorrhagic fever compared with serum samples from healthy persons. The findings suggest that cytokines produced during dengue hemorrhagic infections could induce alterations in the vascular endothelium, which may play a fundamental role in the pathophysiology of dengue.