Consequences of interspecies antennal imaginal disc transplantation on organization of olfactory glomeruli and pheromone blend discrimination.
Consequences of interspecies antennal imaginal disc transplantation on organization of olfactory glomeruli and pheromone blend discrimination.
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种间触角成虫盘移植对嗅觉肾小球组织和信息素混合辨别的影响。
DOI：
10.1002/cne.10890
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
LinnJr,CharlesE
中科院分区：
文献类型：
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作者：
Vickers,NeilJ;Poole,Kathy;LinnJr,CharlesE
将触角影像盘移植到两种不同日蛾种Heliothis virescens（HV）和helicoverpa zea（HZ）的雄性幼虫之间。这些物种的雄性对不同的信息素混合物有反应，具有不同的外周和中枢嗅觉神经元特异性，以及在特殊的雄性大肾小球复合物（MGC）中不同的触角叶嗅肾小球排列方式。在蛹发育和成虫羽化后，研究人员在风洞中检测了单侧（其中一个触角盘完好无损）和双侧触角移植的雄性果蝇对两种物种的信息素混合物的反应，以确定它们区分这两种信号的能力。然后在这些个体中检查了种间移植对飞蛾大脑初级嗅觉中心的变质后发育影响。行为测试表明，两种类型的单侧移植继续表现出逆风风向飞行到正常受体混合体，偶尔飞行到供体混合体。相比之下，无论移植方向如何，双侧移植都倾向于hvpheromone混合物，每种类型的一些雄性也对hzblend有反应。MGC的神经解剖评价显示，73%的hzv供体移植和56%的反向移植诱导了MGC肾小球的供体排列。令人惊讶的是，一些V‐Z双侧移植雄性对hvandhzpheromone混合物都有反应，并且具有两种ohvmgc结构。这种行为结果是出乎意料的，因为对hvblend的反应是由通常对hz雄性拮抗的输入介导的，而正常的hvtenna缺乏能够对hzblend的基本次要信息素成分做出反应的嗅觉受体神经元。这些数据表明，调节外周嗅觉受体神经元表达的发育途径和物种特异性嗅觉信息的肾小球加工具有可塑性。[j] .中国生物医学工程学报，2006,31(2):377 - 388。©2003 Wiley‐Liss, Inc。
The antennal imaginal disc was transplanted between male larvae of two different heliothine moth species,Heliothis virescens(HV) andHelicoverpa zea(HZ). Males of these species respond to distinct pheromone blends, have different peripheral and central olfactory neuron specificities, as well as distinct arrangements of antennal lobe olfactory glomeruli, in the specialized male macroglomerular complex (MGC). After pupal development and adult eclosion, unilateral (with one antennal disc left intact) and bilateral antennal transplant males were assayed in a wind tunnel to both species' pheromone blends to determine their ability to discriminate between the two signals. The postmetamorphic developmental effects of interspecific transplantation upon the primary olfactory centers in the moth brain were then examined in these same individuals. Behavioral tests showed that both types of unilateral transplant continued to exhibit upwind anemotactic flight to the normal recipient blend with occasional flights to the donor blend. In contrast, bilateral transplants preferred theHVpheromone blend regardless of the direction of transplant, with some males of each type also responding to theHZblend. Neuroanatomic evaluation of the MGC revealed that the donor arrangement of MGC glomeruli was induced in 73%HZdonor toHVrecipient transplants and 56% of the reciprocal transplant. Surprisingly, several V‐Z bilateral transplant males responded to bothHVandHZpheromone blends and had twoHVMGC structures. This behavioral outcome was unexpected, because responses to theHVblend are mediated by inputs that are normally antagonistic toHZmales and the normalHVantenna lacks olfactory receptor neurons capable of responding to the essential minor pheromone component of theHZblend. These data indicate a plasticity in developmental pathways regulating the expression of peripheral olfactory receptor neurons and in the glomerular processing of species‐specific olfactory information. J. Comp. Neurol. 466:377–388, 2003. © 2003 Wiley‐Liss, Inc.