Coordinated clearance of periciliary liquid and mucus from airway surfaces
Coordinated clearance of periciliary liquid and mucus from airway surfaces
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DOI：
10.1172/jci2687
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发表时间：
1998-09-15
影响因子：
15.9
通讯作者：
Boucher, RC
中科院分区：
文献类型：
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作者：
Matsui, H;Randell, SH;Boucher, RC
气道表面液体由粘液和下面的水样纤毛周液体（PCL）组成。与充分描述的粘液通过纤毛作用沿气道表面沿着轴向运输相反，理论分析预测PCL几乎是静止的。荧光微球和光活化荧光染料的常规和共聚焦显微镜与表现出自发的、放射状粘膜纤毛转运的分化良好的人气管支气管上皮细胞培养物一起使用，以研究粘液和PCL的运动。这些研究表明，整个PCL以与粘液大致相同的速率转运，分别为39.2+/-4.7和39.8+/-4.2 μ m/sec。去除粘液层使PCL转运减少> 80%，至4.8+/-0.6 μ m/sec，该值接近于从纤毛搏动周期的理论分析预测的值。因此，PCL的快速移动依赖于粘液的运输。粘液依赖性PCL运输在空间上是均匀的，并且超过了与上覆粘液层的纯摩擦耦合的预期速率;因此，纤毛混合最有可能加速从粘液到PCL的动量扩散。PCL沿着气道上皮表面的头向运动使得这种粘液驱动的运输成为健康和疾病中肺中盐和水生理学的重要组成部分。
Airway surface liquid is comprised of mucus and an underlying, watery periciliary liquid (PCL). In contrast to the well-described axial transport of mucus along airway surfaces via ciliary action, theoretical analyses predict that the PCL is nearly stationary. Conventional and confocal microscopy of fluorescent microspheres and photoactivated fluorescent dyes were used with well-differentiated human tracheobronchial epithelial cell cultures exhibiting spontaneous, radial mucociliary transport to study the movements of mucus and PCL, These studies showed that the entire PCL is transported at approximately the same rate as mucus, 39.2+/-4.7 and 39.8+/-4.2 mu m/sec, respectively. Removing the mucus layer reduced PCL transport by > 80%, to 4.8+/-0.6 mu m/sec, a value close to that predicted from theoretical analyses of the ciliary beat cycle. Hence, the rapid movement of PCL is dependent upon the transport of mucus. Mucus-dependent PCL transport was spatially uniform and exceeded the rate expected for pure frictional coupling with the overlying mucus layer; hence, ciliary mixing most likely accelerates the diffusion of momentum from mucus into the PCL. The cephalad movement of PCL along airway epithelial surfaces makes this mucus-driven transport an important component of salt and water physiology in the lung in health and disease.