Characterizing viscoelasticity of unhydrolyzed chicken sternal cartilage extract suspensions: Towards development of injectable therapeutics formulations
Characterizing viscoelasticity of unhydrolyzed chicken sternal cartilage extract suspensions: Towards development of injectable therapeutics formulations
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DOI：
10.1016/j.jmbbm.2017.04.025
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发表时间：
2017-08-01
影响因子：
3.9
通讯作者：
Kothapalli, Chandrasekhar
中科院分区：
文献类型：
--
作者：
Hama, Brian;Mahajan, Gautam;Kothapalli, Chandrasekhar
软骨提取物的外源递送正在被探索作为膝关节炎治疗的有希望的候选者，因为它仿生天然软骨组织特征。在这项研究中，我们报告了由未水解的增稠胸骨提取物粉末构成的水悬浮液的流变学特征。评估了颗粒尺寸（原始与研磨）、悬浮液（水与 PBS）和温度（37 摄氏度与 4 摄氏度）对基于胸骨提取物的颗粒悬浮液的粘弹性的影响。结果表明，随着剪切速率 (gamma) 的增加，这些悬浮液表现出剪切稀化特性，而悬浮液的粘度 (eta)、储存模量 (G') 和损耗模量 (G") 随着颗粒负载量 (phi: 2.5-10 wt%) 的增加而增加。通过研磨减小收到的颗粒尺寸，会降低悬浮液的 G'、G 和 eta，并增加 eta 对这些性能的影响，这可能是由于改进的颗粒填充所致。用水代替水PBS 对流变特性没有显着影响，但温度从 37°C 降低至 4°C 增加了悬浮液的 G'、G" 和 eta，并降低了粉末负载对粘弹性的影响。悬架的时间依赖性响应是粘弹性材料的典型特征，其特征在于最终应力（应力松弛）或应变（蠕变）的渐近方法。结果符合蠕变幂律模型、应力指数衰减的一般松弛模型、流动曲线的 Carreau-Yasuda 模型以及确定最大粉末负载 (phi(m)) 的双参数 Liu 模型。在这些悬浮液中颗粒与颗粒相互作用所涉及的各种力中，静电力似乎占主导地位。这些悬浮液的粘弹性性质的这种表征将有助于配制用于体内应用的基于稳定的可注射软骨提取物的治疗剂。
Exogenous delivery of cartilage extract is being explored as a promising candidate for knee arthritis treatment as it biomimics native cartilage tissue characteristics. In this study, we report on the rheological characterization of aqueous suspensions constituted from a powdered form of unhydrolyzed thicken sternum extract. The effect of particle size (as-received vs. milled), suspension fluid (water vs. PBS), and temperature (37 degrees C vs. 4 degrees C), on the viscoelastic properties of the sternum extract based particulate suspensions were evaluated. Results showed that these suspensions exhibit shear-thinning characteristics as shear rate (gamma) increases, while viscosity (eta), storage (G'), and loss (G") moduli of the suspensions increased with increasing particulate loading (phi: 2.5-10 wt%). Reducing the as-received particle size by milling decreased G', G, and eta of the suspensions and increased the influence of eta on these properties, possibly due to improved particle packing. Replacing water with PBS had no significant effect on the rheological properties, but temperature reduction from 37 degrees C to 4 degrees C increased G', G", and eta of the suspensions and lowered the impact of powder loading on viscoelastic properties. The suspension's time-dependent response was typical of viscoelastic materials, characterized by an asymptotical approach to a final stress (stress relaxation) or strain (creep). Results were fit to a power-law model for creep, a general relaxation model for exponential decay in stress, Carreau-Yasuda models for flow curves, and a two-parameter Liu model to identify the maximum powder loading (phi(m)). Among the various forces involved in particle-particle interactions within these suspensions, electrostatic forces appeared to dominate the most. Such characterization of the viscoelastic nature of these suspensions would help in formulating stable injectable cartilage extract based therapeutics for in vivo applications.