Utilization of Air-Liquid Interface Cultures as an In Vitro Model to Assess Primary Airway Epithelial Cell Responses to the Type 2 Cytokine Interleukin-13
Utilization of Air-Liquid Interface Cultures as an In Vitro Model to Assess Primary Airway Epithelial Cell Responses to the Type 2 Cytokine Interleukin-13
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DOI：
10.1007/978-1-4939-7896-0_30
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Seibold, Max A.
中科院分区：
文献类型：
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作者：
Everman, Jamie L.;Rios, Cydney;Seibold, Max A.
气道上皮排列在呼吸道上，提供主要的保护屏障，防止吸入性损害，包括有毒环境物质和微生物。气道上皮在调节气道免疫应答中也起着关键作用。气道上皮对2型细胞因子白介素-13 （IL-13）的反应对哮喘患者气道炎症、粘液产生和气道高反应性至关重要。需要建立相应的人气道上皮原代细胞模型来研究il -13介导的气道上皮效应的生物学机制。在这里，我们用体外气液界面（ALI）培养模型系统描述了分化的人气道粘膜纤毛上皮的产生。我们还描述了用IL-13刺激这种培养模型的方法，并收集细胞和生物分子来询问气道上皮IL-13反应的细胞和分子方面。
The airway epithelium lines the respiratory tract and provides the primary protective barrier against inhalational insults including toxic environmental substances and microorganisms. The airway epithelium also plays a critical role in regulating airway immune responses. The airway epithelial response to the type 2 cytokine, interleukin-13 (IL-13), is critical to airway inflammation, mucus production, and airway hyperresponsiveness present in asthma. Relevant primary cell models of the human airway epithelium are needed to investigate the biology of IL-13-mediated airway epithelial effects. Here, we describe the generation of a differentiated mucociliary human airway epithelium using an in vitro air-liquid interface (ALI) culture model system. We also describe methods to stimulate this culture model with IL-13 and harvest cells and biomolecules to interrogate cellular and molecular aspects of the airway epithelial IL-13 response.