Distribution of cholesterol and galactosylceramide in rat cerebellar white matter
Distribution of cholesterol and galactosylceramide in rat cerebellar white matter
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DOI：
10.1016/j.bbalip.2006.02.021
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发表时间：
2006-03-01
影响因子：
4.8
通讯作者：
Mansson, Jan-Eric
中科院分区：
文献类型：
--
作者：
Boerner, Katrin;Nygren, Hakan;Mansson, Jan-Eric
利用配备Bi⁺离子簇枪的飞行时间二次离子质谱仪（TOF - SIMS）对大鼠小脑的白质和内颗粒层进行了分析。样本通过高压冷冻、冷冻断裂和冷冻干燥，或者通过骤冷和低温恒温器切片来制备。所鉴定和定位的化学物质包括：钠、钾、磷酸胆碱、胆固醇以及半乳糖神经酰胺（GalC），其碳链长度有C18:0（N - 硬脂酰 - 半乳糖神经酰胺）和C24:0（N - 木蜡酰半乳糖神经酰胺）以及CH24:0（羟基 - 木蜡酰半乳糖神经酰胺）。我们报道了有关白质中髓鞘组织的新发现。一个是富含胆固醇、带状的10 - 20μm区域，该区域不含Na⁺和K⁺。第二个发现是GalC C18和GalC C24相对于这些区域的不同分布，其中GalC C18定位在富含胆固醇的区域，GalC C24定位在富含Na⁺/K⁺的区域。GalC的分布呈大小均匀的0.8 - 1.5μm的小斑点。高压冷冻的样本制备方法使得能够在组织样本中对钠和钾进行单独定位。（c）2006爱思唯尔有限公司。保留所有权利。
White matter and the inner granular layer of rat cerebellum was analysed by imaging time-of-flight secondary-ion mass spectrometry (TOF-SIMS) equipped with a Bi+ ion cluster gun. Samples were prepared by high pressure freezing, freeze-fracturing and freeze drying or by plunge freezing and cryostat sectioning. The identified and localized chemical species were: sodium, potassium, phosphocholine, cholesterol and galactosylceramide (Ga1C) with carbon chain lengths C18:0 (N-stearoyl-galactosylcerai-nide) and C24:0 (N-lignoceroylgalactosylceramide) with CH24:0 (hydroxy-lignoceroylgalactosylceramide). We report new findings regarding the organization of myelin in white matter. One is cholesterol-rich, ribbon-shaped 10-20 mu m areas excluding Na+ and K+. The second finding is the different distribution of Ga1C C18 and Ga1C C24 in relation to these areas, where Ga1C C18 was localized in cholesterol-rich areas and Ga1C C24 was localized in Na/K-enriched areas. The distribution of Ga1C was in small spots, homogeneous in size, of 0.8-1.5 mu m. Sample preparation with high pressure freezing allowed separate localization of sodium and potassium in tissue samples. (c) 2006 Elsevier B.V. All rights reserved.