Comparative Evaluation of Antimicrobial Efficacy of Commercially Available Herbal Dentifrices against Selected Putative Oral Microorganisms: An In Vitro Study
Comparative Evaluation of Antimicrobial Efficacy of Commercially Available Herbal Dentifrices against Selected Putative Oral Microorganisms: An In Vitro Study
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DOI：
10.7860/jcdr/2020/43888.13669
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发表时间：
2020-04-01
影响因子：
0.2
通讯作者：
Kale, Apurva
中科院分区：
文献类型：
--
作者：
Palekar, Jayesh;Zope, Sameer Anil;Kale, Apurva
前言：牙菌斑积聚已被确定为龋齿、牙周病和口臭的主要原因。在世界范围内，广泛的市售非草药和草药牙膏与牙刷一起沿着用作牙菌斑控制中的机械辅助物。由于一些不良的副作用与非草药牙膏有一个越来越受欢迎的草药牙膏之间的一般population.Aim：评估和比较抗菌功效的三个市售草药牙膏对选定的假定致龋，牙周和真菌微生物。材料和方法：对苏丹塔牙膏、丹康蒂牙膏、高露洁汽巴卡牙膏对沙门氏菌纯培养标准菌株的抗菌效果进行了体外评价。变形菌、白色念珠菌、牙龈卟啉单胞菌、A.放线菌属和T.所有选定的微生物均在特定培养基上传代培养。然后使用标准圆盘扩散法在全强度下一式三份地测试牙膏的抗菌功效。通过测量含有牙膏的圆盘周围以毫米为单位的抑菌区来评价抗菌功效。计算所有草药牙膏的抑菌圈的平均值和标准差，并使用单因素方差分析，然后使用Tukey事后检验分析不同组之间的差异。结果：与高露洁Cibaca Vedshakti牙膏相比，Sudanta和Dant Kanti牙膏对大多数测试微生物表现出更高的抗菌活性。三种牙膏的组间比较表明，丹特康帝牙膏在抑制S。Mutans和C.与Sudanta和Colgate Cibaca Vedshakti相比，Sudanta牙膏对白念珠菌的效果显著更好（p=0.05）。Actinomycetemcomitans和T.连翘比高露洁Cibaca Vedshakti牙膏和Dant kanti（p=0.05）。A. Actinomycetemcomitans和T.连翘对高露洁Cibaca Vedshakti牙膏有抵抗力。然而，高露洁Cibaca Vedshakti与Sudanta（p=0.05）和Dant kanti（p>0.05）相比，对牙龈卟啉单胞菌表现出显著的抗菌活性。Actinomycetemcomitans和T.连翘丹特康帝牙膏对选定的致龋和真菌微生物表现出更好的抗菌活性：S。变种人和白色念珠菌。而高露洁Cibaca Vedshakti牙膏对牙龈卟啉单胞菌的抗菌活性最高。
Introduction: Dental plaque accumulation has been identified as the primary cause of dental caries, periodontal diseases, and halitosis. Worldwide, a wide range of commercially available non-herbal and herbal dentifrices are used along with tooth brush as mechanical aids in plaque control. Owing to some undesirable side effects associated with non-herbal toothpastes there has been an increased popularity of herbal toothpaste among the general population.Aim: To assess and compare antimicrobial efficacy of three commercially available herbal toothpastes against selected putative cariogenic, periodontal and fungal microorganisms.Materials and Methods: An in vitro assessment of antimicrobial efficacy of Sudanta, Dant Kanti, Colgate Cibaca Vedshakti toothpastes was done against pure culture standard strains of S. mutans, C albicans, P. gingivalis, A. Actinomycetemcomitans and T.forsythia. All the selected microorganisms were sub-cultured on specific culture media. Antimicrobial efficacy of the toothpastes was then tested in triplicate at full strength using standard disc diffusion method. The antimicrobial efficacy was evaluated by measuring the zones of inhibition in millimetres surrounding disc containing the toothpastes. Means and standard deviations of the inhibitory zones were calculated for all herbal toothpastes and analysed using one-way ANOVA followed by Tukey's post-hoc tests for differences amongst the different groups. The p-value =0.05 was considered statistically significant.Results: Sudanta and Dant Kanti toothpastes demonstrated higher antimicrobial activity against majority of the test microorganisms as compared to Colgate Cibaca Vedshakti toothpaste. Intergroup comparisons among the three toothpastes demonstrated that Dant Kanti toothpaste was significantly better in inhibiting S. Mutans and C. Albicans than Sudanta and Colgate Cibaca Vedshakti (p=0.05) while Sudanta toothpaste was significantly more effective against A. Actinomycetemcomitans and T. Forsythia than Colgate Cibaca Vedshakti toothpaste and Dant kanti (p=0.05). A. Actinomycetemcomitans and T. Forsythia were resistant to Colgate Cibaca Vedshakti toothpaste. However, Colgate Cibaca Vedshakti exhibited substantial antimicrobial activity against P. Gingivalis when compared against Sudanta (p=0.05) and Dant kanti (p>0.05).Conclusion: Results of the present study revealed that Sudanta demonstrated better antimicrobial activity against selected putative periodontal microorganisms: A. Actinomycetemcomitans and T. Forsythia. Dant kanti toothpaste demonstrated better antimicrobial activity against selected putative cariogenic and fungal microorganisms: S. Mutans and C.Albicans. Whereas Colgate Cibaca Vedshakti toothpaste exhibited highest antimicrobial activity against P.gingivalis.