COLLAGEN TYPE-I, TYPE-III AND TYPE-V IN HUMAN-EMBRYONIC AND FETAL SKIN
COLLAGEN TYPE-I, TYPE-III AND TYPE-V IN HUMAN-EMBRYONIC AND FETAL SKIN
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DOI：
10.1002/aja.1001750409
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发表时间：
1986-04-01
影响因子：
--
通讯作者：
MADRI, JA
中科院分区：
文献类型：
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作者：
SMITH, LT;HOLBROOK, KA;MADRI, JA
人类皮肤的真皮从侧板中胚层发育而来，并在妊娠前三个月结束时以成人型模式建立。本研究对妊娠5~26周的胚胎和胎儿真皮中胶原基质的结构、生化和免疫细胞化学进行了研究。5周时的真皮含有覆盖在间充质细胞表面的细小的个别胶原纤维。随着年龄的增长，细胞外间隙中胶原基质的含量增加。纤维直径增大，更多的纤维结合成纤维束。到15周时，乳头状区和网状区被识别。直径更大的纤维、更大的纤维、更密集的胶原堆积和更少的细胞将较深的网状区与位于表皮下的更细的、更多细胞的乳头区区分开来。免疫组织化学染色光镜观察I、III、V型胶原的分布；免疫金标记法在超微结构水平上观察I、III、V型胶原的分布。免疫过氧化物酶检测发现，无论胎龄如何，I型和III型均分布于真皮和真皮下结缔组织，真皮-表皮交界处染色增强。III型和V型胶原染色主要集中在血管周围。V型胶原也定位于表皮的基底层和周层细胞。免疫扫描分析发现，I型和III型与胶原纤维结合，V型定位于真皮细胞表面，范围较小，与纤维结合。胎儿皮肤提取液中I型、III型和V型胶原相对含量的生化分析结果与免疫过氧化物酶数据一致。I型胶原含量为70-75%，III型胶原含量为18-21%，V型胶原含量为6-8%，而成人皮肤中I型胶原含量为89-90%，III型胶原含量为8-11%，V型胶原含量为2-4%。胎儿皮肤中III型和V型胶原的丰富可能在一定程度上反映了血管和神经相关胶原与真皮纤维胶原的比例。随着年龄的增长，真皮纤维胶原的积累将增加I型胶原的估计比例，尽管真皮胶原纤维中III型与I型的比例在所有年龄可能是相似的。
The dermis of human skin develops embryonically from lateral plate mesoderm and is established in an adult-like pattern by the end of the first trimester of gestation. In this study the structure, biochemistry, and immunocytochemistry of collagenous matrix in embryonic and fetal dermis during the period of 5 to 26 weeks of gestation was investigated. The dermis at five weeks contains fine, individual collagen fibrils draped over the surfaces of mesenchymal cells. With increasing age, collagen matrix increases in abundance in the extracellular space. The size of fibril diameters increases, and greater numbers of fibrils associate into fiber bundles. By 15 weeks, papillary and reticular regions are recognized. Larger-diameter fibrils, larger fibers, denser accumulations of collagen, and fewer cells distinguish the deeper reticular region from the finer, more cellular papillary region located beneath the epidermis. The distribution of collagen types I, III, and V were studied at the light microscope level by immunoperoxidase staining and at the ultrastructural level by transmission (TEM) and scanning electron microscopy (SEM) with immunogold labeling. By immunoperoxidase, types I and III were found to be evenly distributed, regardless of fetal age, throughout the dermal and subdermal connective tissue with an intensification of staining at the dermal-epidermal junction (DEJ). Staining for types III and V collagen was concentrated around blood vessels. Type V collagen was also localized in basal and periderm cells of the epidermis. By immuno-SEM, types I and III were found associated with collagen fibrils, and type V was localized to dermal cell surfaces and a more limited extent with fibrils. The results of biochemical analyses for relative amounts of types I, III, and V collagen in fetal skin extracts were consistent with immunoperoxidase data. Type I collagen was 70-75%, type III collagen was 18-21%, and type V was 6-8% of the total of these collagens at all gestational ages tested, compared to 89-90% type I, 8-11% type III, and 2-4% type V in adult skin. The enrichment of both types III and V collagen in fetal skin may reflect in part the proportion of vessel- and nerve-associated collagen versus dermal fibrillar collagen. The accumulation of dermal fibrillar collagen with increasing age would enhance the estimated proportion of type I collagen, even though the ratios of type III to I in dermal collagen fibrils may be similar at all ages.