G-strand overhangs on telomeres in telomerase-deficient mouse cells
G-strand overhangs on telomeres in telomerase-deficient mouse cells
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DOI：
10.1093/nar/27.20.3964
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发表时间：
1999-10-15
影响因子：
14.9
通讯作者：
Greider, CW
中科院分区：
文献类型：
--
作者：
Hemann, MT;Greider, CW
真核染色体的端粒含有 3' 突出端，这被认为对于维持适当的染色体末端结构和功能至关重要。我们研究了哺乳动物细胞中产生这些 G 链突出端对端粒酶活性的要求。通过对端粒酶 RNA 成分缺陷小鼠的组织和培养细胞进行非变性凝胶杂交，我们发现在端粒酶活性缺失的情况下存在 G 链突出端。悬垂信号强度的定量显示，相对于野生型，端粒酶缺陷细胞没有显着减少。这些结果支持产生 G 链突出端的不依赖端粒酶的机制。
Telomeres of eukaryotic chromosomes contain 3' overhangs which are thought to be essential for the maintenance of proper chromosome end structure and function. We examined the requirement for telomerase activity for the generation of these G-strand overhangs in mammalian cells. Using nondenaturing in-gel hybridization to both tissue and cultured cells from mice deficient for the telomerase RNA component, we found that G-strand overhangs exist in the absence of telomerase activity. Quantitation of overhang signal intensity showed no significant reduction in telomerase-deficient cells relative to wild-type. These results support a telomerase-independent mechanism for generating G-strand overhangs.