Antivenomics and venom phenotyping: A marriage of convenience to address the performance and range of clinical use of antivenoms
Antivenomics and venom phenotyping: A marriage of convenience to address the performance and range of clinical use of antivenoms
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DOI：
10.1016/j.toxicon.2009.12.015
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发表时间：
2010-12-15
期刊：
影响因子：
2.8
通讯作者：
Calvete, Juan J.
中科院分区：
文献类型：
--
作者：
Calvete, Juan J.
不同属蛇毒液中存在的同一蛋白质家族的毒素通常具有相同的抗原决定簇。这种情况的一个实际结果是，有可能在免疫学上合理的基础上配制毒液混合物，以产生针对多种物种的抗蛇毒血清。深入了解不同地理来源的蛇毒液抗原成分的种间和种内变异是设计新型多价泛属抗蛇毒血清的关键。这篇综述说明了蛋白质组学方案（“毒液组学”和“抗毒液组学”）如何帮助评估抗蛇毒血清对同源和异源毒液的交叉反应性，从而确定临床应用的范围。最近的研究表明，进化趋势知识以及毒液表型分析如何对设计用于免疫的毒液混合物产生影响，旨在生产泛美洲抗蛇毒血清。 (C) 2009 Elsevier Ltd. 保留所有权利。
Toxins from the same protein family present in venoms from snakes belonging to different genera often share antigenic determinants. A practical consequence of this circumstance is that it might be possible to formulate on an immunologically sound basis a mixture of venoms for generating antivenoms against a wide range of species. A deep insight into the inter- and intraspecific variation of the antigenic constituents of venoms from snakes of different geographic origin represents the key for designing novel polyvalent pan-generic antivenoms. This review illustrates how proteomic protocols ('venomics' and 'anti-venomics') can aid in assessing the crossreactivity of antivenoms against homologous and heterologous venoms, establishing thus the range of clinical application. Recent work showing how the knowledge of evolutionary trends along with venom phenotyping may have an impact in designing a mixture of venoms for immunization aimed to produce a pan-American anti-crotalic antivenom is discussed. (C) 2009 Elsevier Ltd. All rights reserved.