Peripheral nerve regeneration through alginate gel: analysis of early outgrowth and late increase in diameter of regenerating axons
Peripheral nerve regeneration through alginate gel: analysis of early outgrowth and late increase in diameter of regenerating axons
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DOI：
10.1007/s00221-002-1173-y
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发表时间：
2002-10-01
影响因子：
2
通讯作者：
Ide, C
中科院分区：
文献类型：
--
作者：
Hashimoto, T;Suzuki, Y;Ide, C
我们先前的研究显示，以共价键交联的藻酸盐凝胶促进猫和大鼠的周围神经再生。本研究分析了植入后2周内的早期阶段和21个月的晚期阶段通过藻酸盐凝胶进行的神经再生。手术后四天，再生轴突生长，而没有施瓦尔细胞投资通过部分降解的藻酸盐凝胶，直接接触藻酸盐没有基底层覆盖。大量肥大细胞浸润到藻酸盐中。术后1 ~ 2周，再生轴突被普通雪旺细胞包围形成小束，周围的一些轴突部分与藻酸盐直接接触。在远端残端，许多许旺细胞在手术后8-14天迁移到藻酸盐中。它们没有基底层。再生的有髓纤维的直径在8周时很小（约1 μ m），但直径增加，具有与手术后21个月的正常神经相似的分布模式。术后12个月，藻酸盐凝胶-比胶原海绵-和纤维蛋白胶植入远端残端的神经再生效果好得多。这些结果表明，藻酸盐凝胶具有良好的生物相容性再生轴突生长和雪旺细胞迁移，再生纤维可以有一个直径一样厚的正常纤维在长期。藻酸盐凝胶是一种很有前途的周围神经再生植入材料。
Our previous study revealed that alginate gel cross-linked with covalent bonds promoted peripheral nerve regeneration in the cat and rat. The present study analyzed nerve regeneration through alginate gel in the early stages within 2 weeks and the late stages up to 21 months after implantation. Four days after surgery, regenerating axons grew without Schwarm cell investment through the partially degraded alginate gel, being in direct contact with the alginate without a basal lamina covering. Numerous mast cells infiltrated into the alginate. One to 2 weeks after surgery, regenerating axons were surrounded by common Schwann cells to form small bundles, with some axons at the periphery being partly in direct contact with alginate. At the distal stump, numerous Schwann cells had migrated into the alginate 8-14 days after surgery. They had no basal laminae. The diameter of regenerated myelinated fibers was small (approximately I pm) at 8 weeks, but increased in diameter, having a distribution pattern similar to that of normal nerve 21 months after surgery. Much better nerve regeneration was found in alginate gel-, than collagen sponge-, and fibrin glue-implanted distal stump 12 months after surgery. These results indicate that alginate gel has good biocompatibility for regenerating axon outgrowth and Schwann cell migration, and that regenerated fibers can have a diameter as thick as that of normal fibers in the long term. Alginate gel is a promising material for use as an implant for peripheral nerve regeneration.