Impact of microdialysis probes on vasculature and dopamine in the rat striatum: A combined fluorescence and voltammetric study
Impact of microdialysis probes on vasculature and dopamine in the rat striatum: A combined fluorescence and voltammetric study
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DOI：
10.1016/j.jneumeth.2008.06.034
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发表时间：
2008-09-30
影响因子：
3
通讯作者：
Michael, Adrian C.
中科院分区：
文献类型：
--
作者：
Mitala, Christina M.;Wang, Yuexiang;Michael, Adrian C.
通过微透析和/或伏安法测量活体动物脑中的细胞外多巴胺是了解正常脑功能和病理的一种途径。然而，先前的报告表明，组织对植入器械的反应可能会影响测量结果。为了解决组织反应的来源及其对纹状体多巴胺系统的影响，将微透析探针放置在麻醉大鼠的纹状体中。通过双标记荧光显微镜获得的图像显示了探针轨道附近的血脑屏障缺血和开放的迹象。血脑屏障的开放通过测定透析液中卡比多巴（一种通常不渗透大脑的药物）的浓度来进一步检查。虽然在脑透析液中回收了carbi-DOPA，但它并没有改变透析液多巴胺水平或诱发多巴胺释放，如通过探针附近的伏安法所测量的。微透析探针也显着减少纹状体内注入犬尿氨酸细胞外多巴胺水平的影响，通过伏安法测量附近的探针。(C)2008 Elsevier B. V.保留所有权利。
Measuring extracellular dopamine in the brain of living animals by means of microdialysis and/or voltammetry is a route towards understanding both normal brain function and pathology. Previous reports, however, suggest that the tissue response to implantation of devices may affect the outcome of the measurements. To address the source of the tissue response and its impact on striatal dopamine systems microdialysis probes were placed in the striatum of anesthetized rats. Images obtained by dual-label fluorescence microscopy show signs of ischemia and opening of the blood-brain barrier near the probe tracks. Opening of the blood-brain barrier was further examined by determining dialysate concentrations of carbi-DOPA, a drug that normally does not penetrate the brain. Although carbi-DOPA was recovered in brain dialysate, it did not alter dialysate dopamine levels or evoked dopamine release as measured by voltammetry near the probes. Microdialysis probes also significantly diminished the effect of intrastriatal infusion of kynurenate on extracellular dopamine levels as measured by voltammetry near the probes. (C) 2008 Elsevier B.V. All rights reserved.