Co-translational mRNA decay in Saccharomyces cerevisiae.
Co-translational mRNA decay in Saccharomyces cerevisiae.
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RNA衰变和转录的速率决定了所有mRNA的稳态水平，两者都可以受到调节。虽然转录调控的细节越来越被理解，但控制mRNA衰变的机制仍不清楚。在酵母中，mRNA降解的主要途径始于去腺苷化，随后是去帽和5 '-3'核酸外切酶消化。重要的是，假设核糖体必须在转录物被破坏之前从mRNA中去除。与这一预测相反，在这里，我们表明，衰变发生时，mRNA与积极翻译核糖体。这些数据表明，核糖体从mRNA上解离并不是衰变的先决条件，我们认为mRNA降解的5 '-3'极性已经进化，以确保最后易位的核糖体能够完成翻译。
The rates of RNA decay and transcription determine the steady state levels of all mRNAs and both can be subject to regulation. While the details of transcriptional regulation are becoming increasingly understood, the mechanism(s) controlling mRNA decay remain unclear. In yeast, a major pathway of mRNA decay begins with deadenylation followed by decapping and 5’-3’ exonuclease digestion. Importantly, it is hypothesized that ribosomes must be removed from mRNA before transcripts are destroyed. Contrary to this prediction, here we show that decay takes place while mRNAs are associated with actively translating ribosomes. The data indicate that dissociation of ribosomes from mRNA is not a prerequisite for decay and we suggest that the 5’-3’ polarity of mRNA degradation has evolved to ensure that the last translocating ribosome can complete translation.
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