Cell-Free Synthesis of SecYEG Translocon as the Fundamental Protein Transport Machinery
Cell-Free Synthesis of SecYEG Translocon as the Fundamental Protein Transport Machinery
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DOI：
10.1007/s11084-014-9389-y
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发表时间：
2014-12-01
影响因子：
2
通讯作者：
Ueda, Takuya
中科院分区：
文献类型：
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作者：
Matsubayashi, Hideaki;Kuruma, Yutetsu;Ueda, Takuya
细胞膜除了作为一种外膜外，还有许多维持细胞生存所不可缺少的功能。大多数这样的功能是由膜嵌入的蛋白质，通过膜整合机制，SecYEG translocon出现。在此，我们通过体外表达相应的基因来合成SecYEG，以研究细胞膜功能化的过程。
The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.