Proliferation and cellular kinetics of villous epithelial cells and M cells in the chicken caecum
Proliferation and cellular kinetics of villous epithelial cells and M cells in the chicken caecum
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鸡盲肠绒毛上皮细胞和M细胞的增殖和细胞动力学
DOI：
10.1046/j.1469-7580.1998.19320233.x
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发表时间：
1998
影响因子：
2.4
通讯作者：
M. Uehara
中科院分区：
文献类型：
--
作者：
T. Takeuchi;H. Kitagawa;T. Imagawa;M. Uehara
成年鸡肠道绒毛上皮细胞和M细胞的增殖部位和细胞动力学尚未阐明。在本研究中，我们使用秋水仙碱处理和检测增殖细胞核抗原（PCNA）来确定鸡盲肠中的增殖位点。还使用溴脱氧尿苷 (BrdU) 作为示踪剂研究了这些细胞的细胞动力学。沿盲肠肠隐窝的整个长度观察到处于有丝分裂期的肠细胞，除了盲肠扁桃体外，在隐窝的中部分布较密集。分布中心位于距隐窝底部距离的 49% 处，位于盲肠顶端的 41% 处。在盲肠扁桃体中，分布中心在距隐窝底部的长型隐窝中为64％，在短型隐窝中为44％。另一方面，除盲肠扁桃体外，PCNA 阳性肠上皮细胞在隐窝底部分布更密集。分布中心位于距隐窝底部36%的基部，37%在身体，34%在顶端。在盲肠扁桃体中，长型隐窝占 54%，短型隐窝占 44%。施用 BrdU 后 1 天，BrdU 标记的肠细胞到达所有盲肠部分的肠绒毛基底。标记的肠上皮细胞的前缘在第 4 天时从绒毛尖端消失，在基部和主体中消失，在顶端消失 3 天。在盲肠扁桃体中，第1天时隐窝口附近出现了BrdU标记的微绒毛上皮细胞和M细胞，隐窝内未观察到BrdU标记的M细胞。此后，几乎所有这些细胞在第 5 天时从滤泡相关上皮 (FAE) 中消失。这些结果表明，M细胞在1天内从其前体细胞转化，并且M细胞的周转时间发生在前体细胞分裂后的4天内。
The proliferation sites and cellular kinetics of villous epithelial cells and M cells in the intestine of the adult chicken have never been clarified. In this study, we determined the proliferation sites in the chicken caecum using colchicine treatment and detection of proliferative cell nuclear antigen (PCNA). The cellular kinetics of these cells were also studied using bromodeoxyuridine (BrdU) as a tracer. Enterocytes in their mitotic period were observed along the entire length of the intestinal crypt of the caecum, with a denser distribution in the middle portion of the crypt, except for the caecal tonsil. The centres of distributions were at 49% of the distance from the bottom of the crypt in the base and 41% in the apex of the caecum. In the caecal tonsil, the centres of distributions were at 64% in the long type of crypt from the bottom of the crypt and at 44% in the short type of crypt. On the other hand, the PCNA‐positive enterocytes were distributed more densely at the bottom of the crypt, except for the caecal tonsil. The centres of distributions were at 36% in the base from the bottom of the crypt, 37% in the body, and 34% in the apex. In the caecal tonsil, they were at 54% in the long type of crypt and 44% in the short type. The BrdU‐labelled enterocytes reached to the basement of the intestinal villi in all caecal portions at 1 d after the BrdU administration. The leading edge of the labelled enterocytes disappeared from the villous tips at 4 d in the base and the body and 3 d in the apex. In the caecal tonsil, the BrdU‐labelled microvillous epithelial cells and the M cells appeared near the orifice of the crypt at 1 d, and BrdU‐labelled M cells were not observed in the crypt. Thereafter, almost all of these cells disappeared at 5 d from the follicle associated epithelium (FAE). These results suggest that M cells are transformed from their precursors within 1 d, and the turnover time for M cells occurs within 4 d after the cell division of the precursors.