CHARACTERIZATION OF G-PROTEIN ALPHA-SUBUNITS IN THE G(Q) CLASS - EXPRESSION IN MURINE TISSUES AND IN STROMAL AND HEMATOPOIETIC-CELL LINES
CHARACTERIZATION OF G-PROTEIN ALPHA-SUBUNITS IN THE G(Q) CLASS - EXPRESSION IN MURINE TISSUES AND IN STROMAL AND HEMATOPOIETIC-CELL LINES
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DOI：
10.1073/pnas.88.22.10049
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发表时间：
1991-11-01
影响因子：
11.1
通讯作者：
SIMON, MI
中科院分区：
文献类型：
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作者：
WILKIE, TM;SCHERLE, PA;SIMON, MI
小鼠G-α-14和G-α-15 cDNA编码异源三聚体鸟嘌呤核苷酸结合蛋白（G蛋白）的不同α亚基。这些α-亚基与G（q）类的成员相关，并与G-α（q）、G-α-11和G-α-16共享某些序列特征，例如不存在百日咳毒素ADP-核糖基化位点。G-α-11和G-α（q）在鼠组织中普遍表达，但G-α-14主要在脾、肺、肾和睾丸中表达，而G-α-15主要限于造血谱系。在造血细胞系中，G-α-11 mRNA在所有测试的细胞系中发现，G-α（q）广泛表达但在大多数T细胞系中未发现，G-α-15主要在骨髓和B细胞谱系中表达，G-α-14在骨髓粘附（基质）细胞、某些早期骨髓细胞和祖B细胞中表达。从对应于G-α-15的两个区域的合成肽产生的多克隆抗血清与在B细胞膜和用G-α-15 cDNA转化的大肠杆菌中表达的42 kDa的蛋白质反应。在小鼠组织和细胞系中观察到的表达模式表明，G（q）类中的每个α-亚基可能参与百日咳毒素不敏感的信号转导途径，这是造血细胞分化和功能的基础。
Murine G-alpha-14 and G-alpha-15 cDNAs encode distinct alpha-subunits of heterotrimeric guanine nucleotide-binding proteins (G proteins). These alpha-subunits are related to members of the G(q) class and share certain sequence characteristics with G-alpha(q), G-alpha-11, and G-alpha-16, such as the absence of a pertussis toxin ADP-ribosylation site. G-alpha-11 and G-alpha(q) are ubiquitously expressed among murine tissues but G-alpha-14 is predominantly expressed in spleen, lung, kidney, and testis whereas G-alpha-15 is primarily restricted to hematopoietic lineages. Among hematopoietic cell lines, G-alpha-11 mRNA is found in all cell lines tested, G-alpha(q) is expressed widely but is not found in most T-cell lines, G-alpha-15 is predominantly expressed in myeloid and B-cell lineages, and G-alpha-14 is expressed in bone marrow adherent (stromal) cells, certain early myeloid cells, and progenitor B cells. Polyclonal antisera produced from synthetic peptides that correspond to two regions of G-alpha-15 react with a protein of 42 kDa expressed in B-cell membranes and in Escherichia coli transformed with G-alpha-15 cDNA. The expression patterns that were observed in mouse tissues and cell lines indicate that each of the alpha-subunits in the G(q) class may be involved in pertussis toxin-insensitive signal-transduction pathways that are fundamental to hematopoietic cell differentiation and function.