Intravital microscopy: new insights into metastasis of tumors
Intravital microscopy: new insights into metastasis of tumors
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DOI：
10.1242/jcs.072728
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发表时间：
2011-02-01
影响因子：
4
通讯作者：
van Rheenen, Jacco
中科院分区：
文献类型：
--
作者：
Beerling, Evelyne;Ritsma, Laila;van Rheenen, Jacco
转移，即细胞从原发性肿瘤扩散到远处形成继发性肿瘤的过程，仍然没有完全理解。虽然组织学技术提供了重要的信息，但它们只能提供静态图像，从而损害了对这一动态过程的解释。活体显微镜（IVM）的新进展，如双光子显微镜，成像室，荧光和荧光共振能量转移成像，最近已被用来可视化的行为，单个转移细胞在亚细胞分辨率超过几天，产生新的和意想不到的见解这一过程。例如，IVM研究表明，肿瘤细胞可以响应于细胞外基质的不同密度而在多种侵袭策略之间切换。此外，其他IVM研究表明，肿瘤细胞迁移和血液进入不仅发生在侵袭性前沿，而且发生在肿瘤块内缺乏完整基底膜的肿瘤相关血管处。在这篇评论中，我们将概述高分辨率IVM技术的最新进展，并讨论IVM在转移领域的一些最新见解。
Metastasis, the process by which cells spread from the primary tumor to a distant site to form secondary tumors, is still not fully understood. Although histological techniques have provided important information, they give only a static image and thus compromise interpretation of this dynamic process. New advances in intravital microscopy (IVM), such as two-photon microscopy, imaging chambers, and multicolor and fluorescent resonance energy transfer imaging, have recently been used to visualize the behavior of single metastasizing cells at subcellular resolution over several days, yielding new and unexpected insights into this process. For example, IVM studies showed that tumor cells can switch between multiple invasion strategies in response to various densities of extracellular matrix. Moreover, other IVM studies showed that tumor cell migration and blood entry take place not only at the invasive front, but also within the tumor mass at tumor-associated vessels that lack an intact basement membrane. In this Commentary, we will give an overview of the recent advances in high-resolution IVM techniques and discuss some of the latest insights in the metastasis field obtained with IVM.