LOCALIZATION OF DOPAMINE D-3 RECEPTORS TO MESOLIMBIC AND D-2 RECEPTORS TO MESOSTRIATAL REGIONS OF HUMAN FOREBRAIN
LOCALIZATION OF DOPAMINE D-3 RECEPTORS TO MESOLIMBIC AND D-2 RECEPTORS TO MESOSTRIATAL REGIONS OF HUMAN FOREBRAIN
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DOI：
10.1073/pnas.91.23.11271
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发表时间：
1994-11-08
影响因子：
11.1
通讯作者：
JOYCE, JN
中科院分区：
文献类型：
--
作者：
MURRAY, AM;RYOO, HL;JOYCE, JN
我们的特点是结合[I-125]阿替普利多巴胺D-2样和D-3样受体在人纹状体的组织切片。多潘立酮、喹吡罗和7-羟基-N，N-二（1-丙基）-2-氨基四氢萘（7-OH-DPAT）对[I-125]阿替必利结合的竞争最适合假设尾状核中有一个位点，但丘脑核中有两个位点。鸟苷5 '-[β，γ-亚氨基]三磷酸对尾状核中[I-125]阿替普利结合的激动剂抑制作用显示出较大的调节作用，但对丘脑核中的抑制作用不明显。在存在7-OH-DPAT（对D-3样位点的选择性为D-2样位点的1000倍）和多潘立酮（对D-2样位点的选择性为D-3样位点的20倍）的情况下，[I-125]阿替普利的结合用于定量人脑区域中D-2样和D-3样受体的数量。D-2样受体和D-3样受体的分布在很大程度上不重叠。在背侧纹状体中，[I-125]阿替普利与D-3样受体的结合可以忽略不计，但集中在与乙酰胆碱酯酶缺乏的纹状体对齐的背侧核和腹侧壳核中的密集结合岛屿中。结合到D-3样受体也丰富的内部苍白球，腹侧苍白球，隔膜，Calleja岛，基底核，杏仁核的杏仁体纹状体过渡核，杏仁核的中央核，腹侧被盖区。在皮质和海马中检测到[I-125]阿替普利与D-2受体结合，但未检测到D-3受体。
We characterized the binding of [I-125]epidepride to dopamine D-2-like and D-3-like receptors in tissue sections of human striatum. The competition for binding of [I-125]epidepride by domperidone, quinpirole, and 7-hydroxy-N,N-di(1-propyl)-2-aminotetralin (7-OH-DPAT) was best fit by assuming one site in the caudate but two sites in nucleus accumbens. Guanosine 5'-[beta,gamma-imido]triphosphate showed a large modulatory influence in agonist inhibition of [I-125]epidepride binding in caudate but not in nucleus accumbens. The binding of [I-125]epidepride in the presence of 7-OH-DPAT (1000-fold selective for D-3-like versus D-2-like sites) and domperidone (20-fold selective for D-2-like versus D-3-like sites) was used to quantify the numbers of D-2-like and D-3-like receptors in areas of human brain. The distribution of D-2-like and D-3-like receptors was largely nonoverlapping. Binding of [I-125]epidepride to D-3-like receptors was negligible in the dorsal striatum but was concentrated in islands of dense binding in the nucleus accumbens and ventral putamen that aligned with acetylcho linesterase-poor striosomes. Binding to D-3-like receptors was also enriched in the internal globus pallidus, ventral pallidum, septum, islands of Calleja, nucleus basalis, amygdalostriatal transition nucleus of the amygdala, central nucleus of the amygdala, and ventral tegmental area. Binding of [I-125]epidepride to D-2 but not D-3 receptors was detected in cortex and hippocampus.