Acute control of the sleep switch in Drosophila reveals a role for gap junctions in regulating behavioral responsiveness.
Acute control of the sleep switch in Drosophila reveals a role for gap junctions in regulating behavioral responsiveness.
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DOI：
10.7554/elife.37105
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发表时间：
2018-08-15
期刊：
影响因子：
7.7
通讯作者：
van Swinderen B
中科院分区：
文献类型：
--
作者：
Troup M;Yap MH;Rohrscheib C;Grabowska MJ;Ertekin D;Randeniya R;Kottler B;Larkin A;Munro K;Shaw PJ;van Swinderen B
在大多数动物中，睡眠是一个动态的过程，涉及到可能对大脑执行多种功能的不同阶段。在启动睡眠功能之前，很可能需要降低动物对外部世界的行为反应，即使动物还醒着。最近对果蝇的研究发现了果蝇中央大脑背部扇形身体(DFB)的睡眠开关，但尚不清楚这些促进睡眠的神经元是否也控制着在睡眠转换期间忽略环境中显著刺激的迫切需要。我们发现，睡眠开关的光基因激活抑制了对机械刺激的行为反应，即使在清醒的苍蝇中也是如此，这表明这些神经元在调节唤醒方面发挥了更广泛的作用。DFB介导的抑制机制及其相关的神经关联需要innexin6的表达，这表明当苍蝇入睡时迫切需要减少感觉感知，这部分是由电突触介导的。
Sleep is a dynamic process in most animals, involving distinct stages that probably perform multiple functions for the brain. Before sleep functions can be initiated, it is likely that behavioral responsiveness to the outside world needs to be reduced, even while the animal is still awake. Recent work in Drosophila has uncovered a sleep switch in the dorsal fan-shaped body (dFB) of the fly’s central brain, but it is not known whether these sleep-promoting neurons also govern the acute need to ignore salient stimuli in the environment during sleep transitions. We found that optogenetic activation of the sleep switch suppressed behavioral responsiveness to mechanical stimuli, even in awake flies, indicating a broader role for these neurons in regulating arousal. The dFB-mediated suppression mechanism and its associated neural correlates requires innexin6 expression, suggesting that the acute need to reduce sensory perception when flies fall asleep is mediated in part by electrical synapses.