Comparison of Recombinant Proteins of Kinesin 39, Heat Shock Protein 70, Heat Shock Protein 83, and Glycoprotein 63 for Antibody Detection of Leishmania martiniquensis Infection
Comparison of Recombinant Proteins of Kinesin 39, Heat Shock Protein 70, Heat Shock Protein 83, and Glycoprotein 63 for Antibody Detection of Leishmania martiniquensis Infection
复制标题
驱动蛋白 39、热休克蛋白 70、热休克蛋白 83 和糖蛋白 63 重组蛋白在马提尼克利什曼原虫感染抗体检测中的比较
DOI：
10.1111/jeu.12415
复制
发表时间：
2017
影响因子：
2.2
通讯作者：
Leelayoova Saovanee
中科院分区：
文献类型：
--
作者：
Siripattanapipong Suradej;Kato Hirotomo;Tan-ariya Peerapan;Mungthin Mathirut;Leelayoova Saovanee
马提尼克利什曼原虫（Leishmaniamartiniquensis）是一种人畜共患的血鞭毛虫，是人和动物皮肤利什曼病（CL）和内脏利什曼病（VL）的病原体。这种生物最初在马提尼克岛报道，现在已成为泰国的一种新兴传染源。人类感染马丁尼氏杆菌的症状病例不断增加。与此同时，这一新物种的无症状感染严重地引发了全国公众对预防和控制疾病传播的卫生意识和关注。基于杜氏利什曼原虫（Leishmania donovanito）抗原的商业rK39试纸条在检测感染的泰国患者中的抗VL抗体方面的不成功的血清学试验鼓励我们进一步探索新的敏感和特异的抗原表位。本研究测定了马丁尼氏杆菌驱动蛋白39（k39）、热休克蛋白70（hsp70）、热休克蛋白83（hsp83）和糖蛋白63（gp63）的基因序列，并表达了重组蛋白，以评价其对检测马丁尼氏杆菌患者血清中抗马丁尼氏杆菌抗体的诊断效率。Western印迹分析的初步结果表明，K39是最敏感的重组蛋白检测L.martiniquensis。此外，该重组蛋白与抗杜氏利什曼原虫和婴儿利什曼原虫的抗体反应，使其成为进一步开发VL通用快速诊断工具的有希望的抗原。
Leishmania martiniquensis, a zoonotic hemoflagellate, is a causative agent of cutaneous (CL) and visceral leishmaniasis (VL) among humans and animals. This organism, first reported in Martinique Island, now has become an emerging infectious agent in Thailand. Symptomatic cases ofL.martiniquensisinfection among humans have continuously increased. In the meantime, asymptomatic infection of this novel species has seriously created national public health awareness and concern to prevent and control disease transmission. The unsuccessful serological test using the commercial rK39 dipstick based on antigen fromLeishmania donovanito detect the antibodies against VL among infected Thai patients has encouraged us to further explore a new sensitive and specific antigenic epitope. In this study, we determined the sequences and expressed recombinant proteins of kinesin 39 (k39), heat shock protein 70 (hsp70), heat shock protein 83 (hsp83), and glycoprotein 63 (gp63) ofL.martiniquensisto evaluate the diagnostic efficiency to detect antibodies againstL. martiniquensisin patient sera. The preliminary results from western blot analysis have suggested that K39 is the most sensitive recombinant protein to detectL.martiniquensis. Moreover, this recombinant protein reacts with antibodies againstL. donovaniandLeishmania infantum, making it a promising antigen for further development of a universal rapid diagnostic tool for VL.