Protection against lethal Japanese encephalitis virus infection of mice by immunization with the highly attenuated MVA strain of vaccinia virus expressing JEV prM and E genes.
Protection against lethal Japanese encephalitis virus infection of mice by immunization with the highly attenuated MVA strain of vaccinia virus expressing JEV prM and E genes.
复制标题
通过使用表达 JEV prM 和 E 基因的高度减毒痘苗病毒 MVA 株进行免疫接种，可预防小鼠致命的日本脑炎病毒感染。
DOI：
10.1016/s0264-410x(98)00156-x
复制
发表时间：
1999
期刊：
影响因子：
5.5
通讯作者：
Moss,B
中科院分区：
文献类型：
--
作者：
Nam,JH;Wyatt,LS;Chae,SL;Cho,HW;Park,YK;Moss,B
将编码日本脑炎病毒（JEV）韩国株膜（prM）和包膜(E)蛋白糖基化前体的基因插入到限制宿主范围、高度减毒和安全性测试的牛痘病毒MVA株的基因组中。在强合成或修饰的H5痘苗病毒启动子下，分离到含有乙脑病毒基因的MVA重组体。免疫荧光显微镜、流式细胞术和聚丙烯酰胺凝胶电泳检测乙脑病毒prM和E蛋白的合成情况。通过不同途径接种和增强MVA重组中的任何一种的小鼠产生JEV中和抗体，其效价与由灭活的JEV疫苗诱导的抗体相当，以及血凝抑制抗体。通过肌肉或腹腔注射2×106infectious单位MVA/JEV重组免疫的小鼠在9周龄时完全免受105LD50JEV攻击。
Genes encoding the glycosylated precursor of the membrane (prM) and envelope (E) proteins of a Korean strain of Japanese encephalitis virus (JEV) were inserted into the genome of the host-range restricted, highly attenuated, and safety-tested MVA strain of vaccinia virus. MVA recombinants containing the JEV genes, under strong synthetic or modified H5 vaccinia virus promoters, were isolated. Synthesis of JEV prM and E proteins was detected by immunofluorescence microscopy, flow cytometry, and polyacrylamide gel electrophoresis. Mice inoculated and boosted by various routes with either of the MVA recombinants produced JEV neutralizing antibodies, that had titres comparable with those induced by an inactivated JEV vaccine, as well as haemagglutination-inhibiting antibodies. Mice immunized with 2×106infectious units of MVA/JEV recombinants by intramuscular or intraperitoneal routes were completely protected against a 105LD50JEV challenge at 9 weeks of age.