Ultraflexible nanoelectronic probes form reliable, glial scar-free neural integration.
Ultraflexible nanoelectronic probes form reliable, glial scar-free neural integration.
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DOI：
10.1126/sciadv.1601966
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发表时间：
2017-02
期刊：
影响因子：
13.6
通讯作者：
Xie C
中科院分区：
文献类型：
--
作者：
Luan L;Wei X;Zhao Z;Siegel JJ;Potnis O;Tuppen CA;Lin S;Kazmi S;Fowler RA;Holloway S;Dunn AK;Chitwood RA;Xie C
亚细胞大小的超柔性电极与活体大脑形成无缝集成，并提供长期可靠的记录。植入式脑电极是与体内单个神经元电连接的唯一手段，但其记录功效和生物相容性对科学和临床应用造成了限制。我们发现，具有亚细胞尺寸、超柔性和细胞手术足迹的纳米电子线（NET）电极形成了可靠的、无胶质瘢痕的神经整合。我们证明了NET电极可以可靠地检测和跟踪单个单元数月;其阻抗、噪声水平、单个单元记录产率和信号幅度在长期植入期间保持稳定。体内双光子成像和死后组织学分析显示，NET探针与局部细胞和血管网络的无缝亚细胞整合，具有完全恢复的毛细血管和完整的血脑屏障，完全没有慢性神经元降解和神经胶质瘢痕。
Subcellular-sized, ultraflexible electrodes form seamless integration with the living brain and afford chronically reliable recording. Implanted brain electrodes construct the only means to electrically interface with individual neurons in vivo, but their recording efficacy and biocompatibility pose limitations on scientific and clinical applications. We showed that nanoelectronic thread (NET) electrodes with subcellular dimensions, ultraflexibility, and cellular surgical footprints form reliable, glial scar–free neural integration. We demonstrated that NET electrodes reliably detected and tracked individual units for months; their impedance, noise level, single-unit recording yield, and the signal amplitude remained stable during long-term implantation. In vivo two-photon imaging and postmortem histological analysis revealed seamless, subcellular integration of NET probes with the local cellular and vasculature networks, featuring fully recovered capillaries with an intact blood-brain barrier and complete absence of chronic neuronal degradation and glial scar.