Subtyping of uropathogenic Escherichia coli according to the pathogenicity island encoding uropathogenic-specific protein:: Comparison with phylogenetic groups
Subtyping of uropathogenic Escherichia coli according to the pathogenicity island encoding uropathogenic-specific protein:: Comparison with phylogenetic groups
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DOI：
10.1111/j.1442-2042.2006.01398.x
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发表时间：
2006-06-01
影响因子：
2.6
通讯作者：
Yamamoto, Shingo
中科院分区：
文献类型：
--
作者：
Kanamaru, Sojun;Kurazono, Hisao;Yamamoto, Shingo
背景：系统发育分析在分子流行病学研究中广泛应用于肠外致病性大肠杆菌的特征。我们最近报道了一个假定的致病岛（PAI），携带尿病特异性蛋白（usp）和usp后的小开放阅读框的独特马赛克结构，提供了PAIusp的四种亚型，按其顺序模式分类。方法：对无并发症尿路感染（膀胱炎194株、肾盂肾炎76株、前列腺炎107株）分离的427株大肠杆菌和50株粪便分离的大肠杆菌进行系统发育分型、PAIusp分型、毒力因子（VF）和O血清组的流行情况进行检测。结果：系统发育组B2和usp阳性菌株在膀胱炎、肾盂肾炎和前列腺炎中均占主导地位（B2分别为80.9%、86.8%和86.9%；usp分别为79.4%、93.4%和88.8%）。此外，每个PAIusp亚型被证明与几个VF基因以及尿路致病性大肠杆菌的几个常见O血清群密切相关。结论：在分子流行病学研究中，PAIusp分型将为系统发育类群B2的大肠杆菌菌株提供额外的信息发现。
Background: Phylogenetic analysis has been used widely to characterize extraintestinal pathogenic Escherichia coli in molecular epidemiological studies. We have recently reported a putative pathogenicity island (PAI), carrying uropathogenic-specific protein (usp) and a unique mosaic structure of small open reading frames following usp, providing four subtypes of PAIusp classified from their sequential patterns.Methods: A total of 427 E. coli isolates from uncomplicated urinary tract infections (194 cystitis, 76 pyelonephritis, and 107 prostatitis) and 50 fecal isolates were examined for phylogenetic grouping and PAIusp subtyping as well as the prevalence of virulence factors (VF) and O serogroups.Results: Both phylogenetic group B2 and usp-positive strains were equally predominant in cystitis, pyelonephritis and prostatitis (B2, 80.9%, 86.8%, and 86.9%; usp, 79.4%, 93.4%, and 88.8%, respectively). Furthermore, each PAIusp subtype was shown to be closely associated with several VF genes as well as several common O serogroups of uropathogenic E. coli.Conclusions: In molecular epidemiological studies, PAIusp subtyping will provide additional informative findings of E. coli strains belonging to phylogenetic group B2.