Effects of inhibition of gonadotropin releasing hormone secretion on the response to novel objects in young male and female sheep
Effects of inhibition of gonadotropin releasing hormone secretion on the response to novel objects in young male and female sheep
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DOI：
10.1016/j.psyneuen.2013.11.005
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发表时间：
2014-02-01
影响因子：
3.7
通讯作者：
Haraldsen, Ira Ronit Hebold
中科院分区：
文献类型：
--
作者：
Robinson, Jane E.;Evans, Neil P.;Haraldsen, Ira Ronit Hebold
本研究调查了使用特定激动剂阻断GnRH受体对雄性和雌性绵羊对围栏中放置的新物体的反应的作用。该研究是对46只同性双胞胎动物进行的系列研究的一部分。其中一人每四周接受一次GnRH激动剂醋酸戈舍瑞林的皮下植入，而另一人则未接受治疗。在青春期前即刻开始着床;雄性在8周时，雌性在28周时（因为青春期的时间是性别特异性的）。为确定激动剂处理对生殖轴的影响，采集血样用于测量雄性动物的睾酮和雌性动物的孕酮。此外，还测定了阴囊的体积。本研究旨在确定在大脑发育的不同阶段（8，28和48周龄）是否存在对新对象的性别差异行为反应，以及这些反应是否会被GnRHa治疗改变。对新对象的方法行为和相互作用进行了监测，因为在测试期间每单位时间的发声次数。GnRHa处理抑制雄性的睾酮浓度和睾丸生长以及雌性的孕酮释放。绵羊断奶前（8周龄）比断奶后（28和48周龄）更显着发声，这表明与其dams.Our目前的研究表明，男性更有可能离开他们的同类接近一个新的对象比女性分离的压力。由于这种行为并没有改变抑制生殖轴，我们认为，虽然性别差异更明显地表现在青春期后的表型，这些可能是在青春期，但主要不是在青春期改变性激素。(C)2013由Elsevier Ltd.出版
This study investigated the actions of blocking the GnRH receptor using a specific agonist on the response of male and female sheep to a novel object placed in their pen. The study is part of a series performed on 46 same sex twin animals. One of the pair received a subcutaneous implant of the GnRH agonist Goserelin acetate every four weeks while the other remained untreated. Implantation began immediately prior to puberty; at 8 weeks in the males and 28 weeks in the females (as timing of puberty is sex specific). To determine the effects of agonist treatment on the reproductive axis blood samples were collected for measurement of testosterone in the males and progesterone in the females. In addition the volume of the scrotum was determined. The present study aimed to determine whether there are sexually differentiated behavioural responses to a novel object at different stages of brain development (8, 28 and 48 weeks of age) and whether these responses are altered by GnRHa treatment. Approach behaviour towards and interactions with the novel object were monitored as was the number of vocalisations per unit time during the test period. GnRHa treatment suppressed testosterone concentrations and testicular growth in the mates and progesterone release in the females. Sheep vocalised significantly more prior to weaning (8 weeks of age) than post weaning (28 and 48 weeks of age) suggesting stress on separation from their dams.Our current study shows that males are more likely to leave their conspecifics to approach a novel object than females. As this behaviour was not altered by suppression of the reproductive axis we suggest that, although sex differences are more obviously expressed in the phenotype after puberty, these may be developed during adolescence but not primarily altered during puberty by sex hormones. (C) 2013 Published by Elsevier Ltd.