Gold Nanoparticles as a Potential Carrier for Transmucosal Vaccine Delivery
Gold Nanoparticles as a Potential Carrier for Transmucosal Vaccine Delivery
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DOI：
10.1166/jbn.2011.1200
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发表时间：
2011-02-01
影响因子：
2.9
通讯作者：
Jadhav, S. S.
中科院分区：
文献类型：
--
作者：
Pokharkar, Varsha;Bhumkar, Devika;Jadhav, S. S.
经黏膜注射疫苗可引起全身和局部免疫反应。然而，开发针对这些免疫活性部位的递送系统仍然是一个实际的挑战。本研究的目的是探讨壳聚糖还原金纳米颗粒（AuNps）经黏膜递送破伤风类毒素疫苗的潜力。利用壳聚糖制备了两种不同的纳米颗粒体系。将破伤风类毒素负载在壳聚糖纳米颗粒上，壳聚糖降低了AuNps。目前的研究清楚地表明，皮下注射TT可引起全身反应，但未引起任何粘膜反应！响应。然而，口服给药后，TT负载的AuNps能够产生明显更高的粘膜反应。综上所述，我们成功地证明了利用壳聚糖还原金纳米颗粒开发经黏膜疫苗制剂的新方法。
The transmucosal administration of vaccines leads to development of systemic as well as local immune response. However, development of delivery systems targeted at these immunologically active sites remains a practical challenge. The objective of the present study was to explore the potential of chitosan reduced gold nanoparticles (AuNps) for the transmucosal delivery of tetanus toxoid vaccine. Two different nanoparticulate systems were developed utilizing chitosan. Tetanus toxoid was loaded on chitosan nanoparticles and chitosan reduced AuNps. The current study clearly demonstrated that subcutaneous TT administration lead to generation of systemic response, but did not elicit any mucosa! response. However TT loaded AuNps were able to generate a significantly higher mucosal response following oral administration. In conclusion, a novel approach utilizing chitosan reduced gold nanoparticles for the development of transmucosal vaccine formulation was successfully demonstrated.