Overview of subcellular fractionation procedures for the yeast Saccharomyces cerevisiae.
Overview of subcellular fractionation procedures for the yeast Saccharomyces cerevisiae.
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DOI：
10.1002/0471143030.cb0307s07
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Emr, S D
中科院分区：
文献类型：
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作者：
Rieder, S E;Emr, S D
本单元概述了适用于酿酒酵母的离心分离程序。的目标，优点，局限性和关键参数进行了讨论，以促进亚细胞分级策略的发展。课程内容包括酵母细胞裂解物的制备、差速离心、密度梯度离心及亚细胞成分的分析。
This unit provides an overview of centrifugation-based fractionation procedures adapted for the yeast Saccharomyces cerevisiae. The goals, merits, limitations, and critical parameters of are discussed in order to facilitate the development of subcellular fractionation strategies. Topics include yeast cell lysate preparation, differential velocity centrifugation, density gradient centrifugation, and the analysis of subcellular fractions.