Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices.
Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices.
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DOI：
10.1039/c5an01610j
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发表时间：
2016-01-21
期刊：
影响因子：
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通讯作者：
Issadore D
中科院分区：
文献类型：
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作者：
Ko J;Carpenter E;Issadore D
在过去的几年中，已经发现源自肿瘤细胞并且可以在血液中循环的纳米级囊泡（即外来体和微囊泡）含有丰富的蛋白质组学和遗传信息，以监测癌症进展、转移和药物功效。然而，使用外泌体和微泡作为生物标志物来改善患者护理受到其小尺寸（30 nm-1 μm）以及其分离和测量所需的大量样品制备的限制。在这篇评论中，我们探讨了微纳米技术在临床样品中分离和检测外泌体和微泡的新用途，以及该技术在癌症监测和诊断中的应用。
In the last several years, nanoscale vesicles that originate from tumor cells and which can be found circulating in the blood (i.e. exosomes and microvesicles) have been discovered to contain a wealth of proteomic and genetic information to monitor cancer progression, metastasis, and drug efficacy. However, the use of exosomes and microvesicles as biomarkers to improve patient care has been limited by their small size (30 nm–1 μm) and the extensive sample preparation required for their isolation and measurement. In this Critical Review, we explore the emerging use of micro and nano-technology to isolate and detect exosomes and microvesicles in clinical samples and the application of this technology to the monitoring and diagnosis of cancer.