Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells
Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells
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DOI：
10.1016/j.bbrc.2007.05.070
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发表时间：
2007-07-27
影响因子：
3.1
通讯作者：
Kusumoto, Kenji
中科院分区：
文献类型：
--
作者：
Kakudo, Natsuko;Shimotsuma, Ayuko;Kusumoto, Kenji
脂肪来源的干细胞(ASCs)根据诱导刺激、特定的生长和因子表现出向多种谱系分化的能力，包括骨、软骨或脂肪。成纤维细胞生长因子-2(成纤维细胞生长因子-2)可促进ASCs向软骨方向分化并抑制其向成骨方向分化，但对其在成脂分化中的作用缺乏深入研究。在这项研究中，我们在细胞和分子水平上证明了由3-异丁基-1-甲基黄嘌呤(IBMX)、地塞米松、胰岛素和消炎痛组成的成脂激素鸡尾酒对ASCs成脂分化的影响。成纤维细胞生长因子-2显著促进人ASCs的成脂分化。此外，在成脂诱导前加入成纤维细胞生长因子-2的培养细胞中，脂肪形成过程中的关键转录因子--过氧化物酶体增殖物激活受体-γ2(PPAR-γ2)的mRNA表达上调。在脂肪组织再生中加入成纤维细胞生长因子-2，提示成纤维细胞生长因子和血管干细胞在脂肪组织再生中有潜在的应用前景。(C)2007 Elsevier Inc.保留所有权利。
Adipose-derived stem cells (ASCs) have demonstrated a capacity for differentiating into a variety of lineages, including bone, cartilage, or fat, depending on the inducing stimuli and specific growth and factors. It is acknowledged that fibroblast growth factor-2 (FGF-2) promotes chondrogenic and inhibits osteogenic differentiation of ASCs, but thorough investigations of its effects on adipogenic differentiation are lacking. In this study, we demonstrate at the cellular and molecular levels the effect of FGF-2 on adipogenic differentiation of ASCs, as induced by an adipogenic hormonal cocktail consisting of 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, insulin, and indomethacin. FGF-2 significantly enhances the adipogenic differentiation of human ASCs. Furthermore, in cultures receiving FGF-2 before adipogenic induction, mRNA expression of peroxisome proliferator-activated receptor gamma 2 (PPAR gamma 2), a key transcription factor in adipogenesis, was upregulated. The results of FGF-2 supplementation suggest the potential applications of FGF-2 and ASCs in adipose tissue regeneration. (c) 2007 Elsevier Inc. All rights reserved.