Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis
Hainanenins: A novel family of antimicrobial peptides with strong activity from Hainan cascade-frog, Amolops hainanensis
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海南宁：来自海南梯级蛙（Amolops hainanensis）的一类具有强活性的新型抗菌肽
DOI：
10.1016/j.peptides.2012.01.014
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发表时间：
2012-02-01
期刊：
影响因子：
3
通讯作者：
Yu, Haining
中科院分区：
文献类型：
--
作者：
Zhang, Songyan;Guo, Huanhuan;Yu, Haining
两栖动物皮肤分泌的抗菌肽是一种重要的天然免疫防御策略。蛙科动物有340余种，其中有近100个家族的AMP含有8 ~ 48个氨基酸残基。本研究从海南溪蛙（Amolops hainanensis）皮肤分泌物中分离纯化了两种新的抗菌肽，并从构建的海南溪蛙皮肤cDNA文库中克隆了31个编码4个家族10种新抗菌肽的cDNA序列。海南种。在这10个AMP中，5个肽代表了一个新的两栖动物AMP家族的原型。根据该种的属名，将其命名为hainanenin-1-5。它们每个由21个氨基酸残基组成，在Cys（15）和Cys（21）之间具有7个残基的C-末端二硫环。合成了其中两个化合物（海南烯-1和5），并对其进行了体外抗菌、溶血和抗氧化活性的筛选。结果表明，海南烯素-1和5对革兰氏阳性菌、革兰氏阴性菌和真菌具有较强的广谱抗菌活性，包括大量临床分离的耐药病原微生物，且抗氧化活性较弱。不希望的是，海南烯-1和5对人红细胞表现出强的溶血活性。海南素的发现及其强大的抗菌活性为抗感染药物的设计提供了新的模板。皇冠版权所有（C）2012由Elsevier Inc.发布。All rights reserved.
Antimicrobial peptides (AMPs) secreted by amphibian skin represent an important innate immune defense strategy. There are more than 340 species in the family of Ranidae worldwidely, and from which nearly 100 families of AMPs comprising between 8 and 48 amino acid (aa) residues have been characterized. In current work, two novel AMPs were purified from the skin secretion of Hainan cascade-frog, Amolops hainanensis, and 31 cDNA sequences encoding 10 novel AMPs belonging to 4 families were cloned from the constructed skin cDNA library of A. hainanensis. Among these 10 AMPs, 5 peptides represent the prototypes of a novel amphibian AMP family. According to the generic name of the species of origin, they were designated as hainanenin-1-5. Each of them consists of 21 aa residues with a C-terminal disulphide loop of 7 residues between Cys(15) and Cys(21). Two of them (hainanenin-1 and 5) were then synthesized and their in vitro activities were screened, including antimicrobial, hemolytic and antioxidant activities. The results showed that hainanenin-1 and 5 possessed strong and broad-spectrum antimicrobial activities against Gram-positive, Gram-negative bacteria and fungi, including a large number of clinically isolated drug-resistant pathogenic microorganisms, and slight antioxidant activity. Undesirably, hainanenin-1 and 5 exhibited strong hemolytic activity on human erythrocytes. The discovery of hainanenins and their great antimicrobial potency provides new templates for anti-infective agent design. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.