Ethidium Bromide Inhibits Mitochondrial Phosphorylating Oxidation
Ethidium Bromide Inhibits Mitochondrial Phosphorylating Oxidation
复制标题
溴化乙锭抑制线粒体磷酸化氧化
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
K. S. Rogers
中科院分区：
文献类型：
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作者：
E. S. Higgins;Brenda L. Dunlavey;Wilson H. Friend;K. S. Rogers
溴化乙啶除与线粒体核酸结合外，在线粒体谷氨酸和琥珀酸呼吸过程中是一种磷酸化抑制剂。彻底洗涤溴化乙啶处理的线粒体并没有减轻抑制作用，也没有显著减少结合染料的数量。与阳离子交换树脂在3°下透析17小时，脱除约97%的结合染料。这恢复了未处理的线粒体的磷酸化能力，这些线粒体也被树脂透析了。由于状态3呼吸减少，状态4不受吖啶染料的影响，并且由于线粒体肿胀和潜在atp酶的释放都没有观察到，那么溴化乙啶不是电子传递抑制剂，也不是氧化磷酸化的解偶联剂。溴化乙锭对代谢过程的抑制可能部分是由于抑制了线粒体ATP的生成。这项工作得到了美国癌症协会机构资助号的部分支持。在- 105。
Summary Ethidium bromide, in addition to combination with mitochondrial nucleic acids, is a phosphorylation inhibitor during glutamate and succinate respiration by mitochondria. Exhaustive washing of ethidium bromide-treated mitochondria did not relieve the inhibition nor significantly decrease the amount of bound dye. Dialysis against a cation exchange resin at 3° for 17 hr removed about 97% of bound dye. This restored phosphorylating capacity to that of untreated mitochondria which had also been dialyzed against the resin. Since state 3 respiration was diminished and state 4 was unaffected by the presence of the acridine dye, and since neither swelling of mitochondria nor release of latent ATPase was observed, then ethidium bromide was not an electron transport inhibitor nor an uncoupler of oxidative phosphorylation. Inhibition of metabolic processes by ethidium bromide may be due in part to depressed generation of mitochondrial ATP. This work was supported in part by an American Cancer Society Institutional Grant No. IN-105.