Comparison of cytokine expression profile during Wallerian degeneration of myelinated and unmyelinated peripheral axons
Comparison of cytokine expression profile during Wallerian degeneration of myelinated and unmyelinated peripheral axons
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DOI：
10.1016/j.neulet.2007.11.003
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发表时间：
2008-01-17
影响因子：
2.5
通讯作者：
Hoeke, Ahmet
中科院分区：
文献类型：
--
作者：
Cheepudomwit, Therapoj;Guzelsu, Emre;Hoeke, Ahmet
已经使用神经横断模型研究了沃勒变性期间细胞因子和趋化因子表达的变化，其导致髓鞘化和非髓鞘化雪旺细胞的去神经支配。非髓鞘化的Remak Schwann细胞对它们的轴突损失的细胞因子和趋化因子反应是未知的。在这项研究中，我们比较了辣椒素诱导的无髓轴突变性后，远端神经中的各种细胞因子和趋化因子的表达谱神经横断引起的沃勒变性。MCP-1，IL-2，IL-6和IL-10的上调在两组中均可见，但IL-1 β和LIF主要在整个神经的沃勒变性中上调，而不是在辣椒素诱导的无髓轴突变性中上调。活化的巨噬细胞反应，如通过艾德-1免疫染色的增加所测量的，与辣椒素处理的神经相比，在横断的坐骨神经中更突出。这些研究结果表明，有髓鞘和非髓鞘雪旺细胞的细胞因子和趋化因子的反应，以损失其轴突的差异，并添加到越来越多的文献，指出雪旺细胞之间更大的异质性。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
Changes in cytokine and chemokine expression during Wallerian degeneration have been studied using nerve transection models, which result in denervation of both myelinating and non-myelinating Schwann cells. Cytokine and chemokine response of non-myelinating Remak Schwann cells to loss of their axons is unknown. In this study, we compared the expression profile of various cytokines and chemokines in distal nerves after capsaicin-induced degeneration of unmyelinated axons to Wallerian degeneration induced by nerve transection. Upregulation of MCP-1, IL-2, IL-6 and IL-10 were seen in both groups but IL-1 beta and LIF were primarily upregulated in Wallerian degeneration of the whole nerve and not in capsaicin-induced degeneration of unmyelinated axons. The activated macrophage response, as measured by an increase in ED-1 immunostaining, was more prominent in the transected sciatic nerves compared to capsaicin-treated nerves. These findings indicate that there are differences in the cytokine and chemokine response of myelinating and non-myelinating Schwann cells to loss of their axons, and add to a growing body of literature that points to greater heterogeneity among Schwann cells. (c) 2007 Elsevier Ireland Ltd. All rights reserved.