Development, characterization and transferability of microsatellite markers for the plant genus Phlox (Polemoniaceae)

Development, characterization and transferability of microsatellite markers for the plant genus Phlox (Polemoniaceae)
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福禄考(Polemoniaceae)植物属微卫星标记的开发、表征和可转移性

DOI:
10.1111/j.1471-8286.2007.01891.x
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发表时间:
2008
影响因子:
7.7
通讯作者:
C. Ferguson
C. Ferguson
中科院分区:
生物学1区
文献类型:
--
作者:
Shannon D. Fehlberg;KRISTEN A. Ford;M. Ungerer;C. Ferguson

文献摘要

被引文献

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为了研究福禄考的多样性和微进化,我们开发了9个多态性微卫星座位。结果表明,在同一群体中,平均等位基因数为10.0 ± 5.1,平均观察杂合度为0.611 ± 0.234,平均期望杂合度为0.769 ± 0.170。这些标记中的大多数在福禄考属的另外11个种中成功扩增,代表了该属的广泛多样性,并且一些也在Polemoniaceae的更远的相关成员中扩增。这些微卫星标记将有助于研究这一重要研究系统的进化过程。
In order to study diversification and microevolution in Phlox, we developed nine polymorphic microsatellite loci. In 20 individuals of Phlox pilosa from a single population, the average number of alleles per locus was 10.0 ± 5.1, and average observed and expected heterozygosities were 0.611 ± 0.234 and 0.769 ± 0.170, respectively. Most of these markers amplified successfully in 11 additional species of Phlox, representing a broad diversity of the genus, and some also amplified in more distantly related members of the Polemoniaceae. These microsatellite markers will be valuable for investigation of evolutionary processes in this important study system.