Impact of plant growth promoting Pseudomonas monteilii PsF84 and Pseudomonas plecoglossicida PsF610 on metal uptake and production of secondary metabolite (monoterpenes) by rose-scented geranium (Pelargonium graveolens cv. bourbon) grown on tannery sludge amended soil
Impact of plant growth promoting Pseudomonas monteilii PsF84 and Pseudomonas plecoglossicida PsF610 on metal uptake and production of secondary metabolite (monoterpenes) by rose-scented geranium (Pelargonium graveolens cv. bourbon) grown on tannery sludge amended soil
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DOI：
10.1016/j.chemosphere.2014.08.001
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发表时间：
2014-12-01
期刊：
影响因子：
8.8
通讯作者：
Patra, Dharani Dhar
中科院分区：
文献类型：
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作者：
Dharni, Seema;Srivastava, Atul Kumar;Patra, Dharani Dhar
从印度坎普尔Jajmau制革污泥污染土壤中分离到两株细菌PsF84和PsF610。16S rRNA基因序列测定和系统发育分析证实，PsF84为假单胞菌，PsF610为舌假单胞菌。以玫瑰香天葵(Pelargonium graveolens cv.)为材料，进行了温室试验。波旁威士忌)种植在用不同比例的制革污泥处理过的土壤中。土泥比分别为100：0、25：75、50：50、75：25和0：100，以评价接种细菌对重金属吸收的影响。该菌株能溶解无机磷，并能产生吲哚乙酸(IAA)和铁载体。与未接种对照相比，菌株PsF84的地上部干生物量增加44%，根系干生物量增加48%，挥发油产量增加43%，叶绿素增加31%。与菌株PsF610相比，分别提高了38%、40%、39%和28%。扫描电子显微镜(SEM)研究表明，铬(VI)的积累导致了根中维管束和贮藏物质的破坏。腺毛(GT)可能是精油合成的主要部位，因此用扫描电子显微镜对其进行了研究。由于其广泛的作用谱，这些菌株可以作为有效的金属隔离和生物接种剂，因为在金属胁迫的土壤中，这些菌株能够产生IAA、铁载体和溶解天葵的磷酸盐。本研究为金属污染土壤的植物修复提供了新的视角。(C)2014爱思唯尔有限公司。保留所有权利。
Bacterial strains PsF84 and PsF610 were isolated from tannery sludge polluted soil, Jajmau, Kanpur, India. 16S rRNA gene sequence and phylogenetic analysis confirmed the taxonomic affiliation of PsF84 as Pseudomonas monteilii and PsF610 as Pseudomonas plecoglossicida. A greenhouse study was carried out with rose-scented geranium (Pelargonium graveolens cv. bourbon) grown in soil treated with tannery sludge in different proportions viz. soil: sludge ratio of 100:0, 25:75, 50:50, 75:25 and 0:100 to evaluate the effects of bacterial inoculation on the heavy metal uptake. The isolates solubilized inorganic phosphorus and were capable of producing indole acetic acid (IAA) and siderophore. The isolate PsF84 increased the dry biomass of shoot by 44%, root by 48%, essential oil yield 43% and chlorophyll by 31% respectively over uninoculated control. The corresponding increase with the isolate PsF610 were 38%, 40%, 39% and 28%, respectively. Scanning electron microscopic (SEM) studies reveal that the Cr(VI) accumulation resulted in breakdown of vascular bundles and sequesters Cr(VI) in roots. The glandular trichomes (GT) were investigated using SEM studies as these glands are probably the main site of essential oil synthesis. Owing to its wide action spectrum, these isolates could serve as an effective metal sequestering and bioinoculants due to the production of IAA, siderophore and solubilization of phosphate for geranium in metal-stressed soil. The present study has provided a new insight into the phytoremediation of metal-contaminated soil. (C) 2014 Elsevier Ltd. All rights reserved.