Effective Photothermal Killing of Pathogenic Bacteria by Using Spatially Tunable Colloidal Gels with Nano-Localized Heating Sources
Effective Photothermal Killing of Pathogenic Bacteria by Using Spatially Tunable Colloidal Gels with Nano-Localized Heating Sources
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DOI：
10.1002/adfm.201403478
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发表时间：
2015-02-04
影响因子：
19
通讯作者：
Sung, Hsing-Wen
中科院分区：
文献类型：
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作者：
Hsiao, Chun-Wen;Chen, Hsin-Lung;Sung, Hsing-Wen
迫切需要治疗皮下脓肿的替代方法，特别是那些与抗生素耐药致病细菌菌株相关的方法，以消除对抗生素的需求。这项工作描述了一种带有自掺杂聚苯胺(PANI)侧链的壳聚糖(CS)衍生物，它可以在水环境中自组装成胶束，并在局部pH升高的驱动下转化为胶体凝胶。这些自掺杂PANI胶束可以用作纳米局域热源，利用近红外光进行远程控制。为了测试CS衍生物作为光热剂的体内效果，将其水溶液直接注射到小鼠感染脓肿的部位。注入的聚合物溶液最终分布在酸性脓肿上，当它到达健康组织的边界时形成胶体凝胶。在808 nm激光治疗后，这种胶体凝胶将近红外光转化为热，导致细菌的热分解，并修复感染的伤口，而不会留下剩余的植入材料。这种方法具有显著的潜力，因为它可以提供具有可调空间稳定性的胶体凝胶，限制对感染部位的局部加热，并减少对周围健康组织的热损害。
Alternative approaches to treating subcutaneous abscessesespecially those associated with antibiotic-resistant pathogenic bacterial strainsthat eliminate the need for antibiotics are urgently needed. This work descibes a chitosan (CS) derivative with self-doped polyaniline (PANI) side chains that can self-assemble into micelles in an aqueous environment and be transformed into colloidal gels in a process that is driven by a local increase in pH. These self-doped PANI micelles can be utilized as nano-localized heat sources, remotely controllable using near-infrared (NIR) light. To test the in vivo efficacy of the CS derivative as a photothermal agent, an aqueous solution thereof is directly injected at the site of infected abscesses in a mouse model. The injected polymer solution eventually becomes distributed over the acidic abscesses, forming colloidal gels when it meets the boundaries of healthy tissues. After treatment with an 808 nm laser, the colloidal gels convert NIR light into heat, causing the thermal lysis of bacteria and repairing the infected wound without leaving residual implanted materials. This approach has marked potential because it can provide colloidal gels with tunable spatial stability, limiting localized heating to the infected sites, and reducing thermal damage to the surrounding healthy tissues.