Med24 and Mdh2 are required for Drosophila larval salivary gland cell death.
Med24 and Mdh2 are required for Drosophila larval salivary gland cell death.
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Med24 和 Mdh2 是果蝇幼虫唾液腺细胞死亡所必需的。
DOI：
10.1002/dvdy.22213
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Thummel,CarlS
中科院分区：
文献类型：
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作者：
Wang,Lei;Lam,Geanette;Thummel,CarlS
类固醇激素蜕皮激素通过转录级联反应触发幼虫组织的快速破坏，最终导致蛋白内表达和半胱天冬酶激活。在这里，我们发现mdh2和med24的突变阻断了半胱天冬酶的裂解和幼虫唾液腺细胞的死亡。mdh2编码一种定位于线粒体的苹果酸脱氢酶。与上述功能一致的是，mdh2突变体的ATP水平显著降低，并积累了晚期柠檬酸循环中间体，这表明细胞死亡缺陷是由能量产生的缺陷引起的。med24编码中介转录辅激活因子复合体的一个组成部分。然而，出乎意料的是，在med24突变体的唾液腺中，关键死亡调控基因的表达是正常的。本研究确定了果蝇变形过程中控制幼虫组织破坏的新机制，并为我们理解类固醇引发的程序性细胞死亡提供了新的方向。科学通报，2009(3):559 - 564。©2010 Wiley‐Liss, Inc。
The steroid hormone ecdysone triggers the rapid destruction of larval tissues through transcriptional cascades that culminate inrprandhidexpression and caspase activation. Here, we show that mutations inMdh2andMed24block caspase cleavage and larval salivary gland cell death.Mdh2encodes a predicted malate dehydrogenase that localizes to mitochondria. Consistent with this proposed function,Mdh2mutants have significantly lower levels of ATP and accumulate late‐stage citric acid cycle intermediates, suggesting that the cell death defects arise from a deficit in energy production.Med24encodes a component of the Mediator transcriptional coactivator complex. Unexpectedly, however, expression of the key death regulator genes is normal inMed24mutant salivary glands. This study identifies novel mechanisms for controlling the destruction of larval tissues duringDrosophilametamorphosis and provides new directions for our understanding of steroid‐triggered programmed cell death. Developmental Dynamics 239:954–964, 2010. © 2010 Wiley‐Liss, Inc.