Osteoblast Production by Reserved Progenitor Cells in Zebrafish Bone Regeneration and Maintenance
Osteoblast Production by Reserved Progenitor Cells in Zebrafish Bone Regeneration and Maintenance
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DOI：
10.1016/j.devcel.2017.10.015
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发表时间：
2017-12-04
影响因子：
11.8
通讯作者：
Kawakami, Atsushi
中科院分区：
文献类型：
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作者：
Ando, Kazunori;Shibata, Eri;Kawakami, Atsushi
哺乳动物不能像在大骨折间隙那样重新形成严重受损的骨骼，而斑马鱼即使在截肢附属物后也能有效地再生骨骼。然而，介导附件再生的成骨细胞的来源是有争议的。几项对斑马鱼的研究表明，成骨细胞是通过损伤部位现有成骨细胞的去分化而产生的，但其他观察结果表明，成骨细胞的新生也会发生。在这项研究中，我们从细胞谱系追踪和消融实验中发现，一组保留在壁龛中的细胞作为成骨细胞祖细胞（OPCs），在鳍的再生中起着重要的作用。除了再生外，OPCs还提供成骨细胞用于正常的骨维持。我们进一步表明，OPCs来源于胚胎体细胞，就像胚胎成骨细胞一样，并从成年斑马鱼的间充质前体中得到补充。我们的研究结果表明，保留祖细胞是斑马鱼骨再生的重要和补充的成骨细胞来源。
Mammals cannot re-form heavily damaged bones as in large fracture gaps, whereas zebrafish efficiently regenerate bones even after amputation of appendages. However, the source of osteoblasts that mediate appendage regeneration is controversial. Several studies in zebrafish have shown that osteoblasts are generated by dedifferentiation of existing osteoblasts at injured sites, but other observations suggest that de novo production of osteoblasts also occurs. In this study, we found from cell-lineage tracing and ablation experiments that a group of cells reserved in niches serves as osteoblast progenitor cells (OPCs) and has a significant role in fin ray regeneration. Besides regeneration, OPCs also supply osteoblasts for normal bone maintenance. We further showed that OPCs are derived from embryonic somites, as is the case with embryonic osteoblasts, and are replenished from mesenchymal precursors in adult zebrafish. Our findings reveal that reserved progenitors are a significant and complementary source of osteoblasts for zebrafish bone regeneration.