Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin-induced diabetic rats
Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin-induced diabetic rats
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DOI：
10.1007/s11418-008-0228-1
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发表时间：
2008-07-01
影响因子：
3.3
通讯作者：
Konuk, Muhsin
中科院分区：
文献类型：
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作者：
Cemek, Mustafa;Kaga, Sadik;Konuk, Muhsin
具有抗糖尿病活性的植物为开发治疗糖尿病的新药提供了重要的资源。本研究对母菊地上部分的降血糖和抗氧化活性进行了研究。链脲佐菌素（STZ; 70 mg/kg，i.p.）中的乙醇提取物（MCE）-糖尿病大鼠分配以下组：假手术组（未接受任何物质）、STZ+蒸馏水组（对照组）、STZ +5 mg/kg格列本脲组、STZ +20 mg/kg MCE组、STZ +50 mg/kg MCE组、STZ +100 mg/kg MCE组。糖尿病大鼠通过管饲法治疗14天。测量餐后血糖水平、丙二醛、还原型谷胱甘肽（GSH）、硝酸盐、亚硝酸盐、抗坏血酸、视黄醇、β-胡萝卜素、超氧化物歧化酶和过氧化氢酶水平，并在所有组中进行免疫组织化学研究。结果表明，STZ可引起氧化应激，影响抗氧化状态。不同剂量的MCE治疗显著降低餐后高血糖和氧化应激，增强抗氧化系统。在组织学研究中，相对于对照组，MCE处理保护了大多数胰岛细胞。结果表明，MCE具有显著的降血糖作用，并以剂量依赖性方式保护STZ糖尿病大鼠的β细胞，减轻高血糖相关的氧化应激。
Plants with antidiabetic activities provide important sources for the development of new drugs in the treatment of diabetes mellitus. In the present study, we investigated possible antihyperglycemic and antioxidative activities of the aerial part of the Matricaria chamomilla L. ethanolic extract (MCE) in streptozotocin (STZ; 70 mg/kg, i.p.)-induced diabetic rats. The following groups were assigned; sham (did not receive any substance), STZ + distilled water (control), STZ + 5 mg/kg glibenclamide, STZ + 20 mg/kg MCE, STZ + 50 mg/kg MCE, STZ + 100 mg/kg MCE. Diabetic rats were treated for 14 days by gavage. Postprandial blood glucose levels, malondialdehyde, reduced glutathione (GSH), nitrate, nitrite, ascorbic acid, retinol, beta-carotene, superoxide dismutase, and catalase levels were measured, and immunohistochemical studies were performed in all of the groups. The obtained data showed that STZ resulted in oxidative stress and affected the antioxidant status. Treatment with different doses of MCE significantly reduced postprandial hyperglycemia and oxidative stress, and augmented the antioxidant system. In histological investigations, MCE treatment protected the majority of the pancreatic islet cells, with respect to the control group. As a result, MCE exhibited significant antihyperglycemic effect and protected beta-cells in STZ-diabetic rats, in a dose-dependent manner, and diminished the hyperglycemia-related oxidative stress.