The latent-TGFβ-binding-protein-1 (LTBP-1) is expressed in the organizer and regulates nodal and activin signaling
The latent-TGFβ-binding-protein-1 (LTBP-1) is expressed in the organizer and regulates nodal and activin signaling
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DOI：
10.1006/dbio.2002.0716
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发表时间：
2002-08-01
影响因子：
2.7
通讯作者：
Brivanlou, AH
中科院分区：
文献类型：
--
作者：
Altmann, CR;Chang, CB;Brivanlou, AH
潜在的tgf - β结合蛋白（LTBP）被认为控制tgf - β在细胞外环境中的可用性。为了深入了解LTBP在早期发育中的潜在作用，我们分离了爪蟾LTBP-1 (xLTBP-1) cDNA。该cDNA编码的蛋白在大小和结构域上都与哺乳动物的LTBP-1相似。此外，我们还发现了xLTBP的一个新的更长的剪接异构体。两种形式的xLTBP的rna都表现出时间调控，较短的转录本在母体中表达。这两个转录本也显示空间调控，并在组织者的背中胚层中发现。在动物帽实验中，LTBP-1增强了激活素和节点的活性。LTBP-1的活性似乎不需要与激活素共价结合，因为在动物帽中添加含有激活素和LTBP-1的培养基增强了激活素的作用。这些结果表明，LTBP-1可能是调节系统的一部分，该系统在原肠胚形成过程中为胚胎背侧的激活素和结建立形态原活性的阈值。(C) 2002 Elsevier Science （USA）。
The latent TGF-beta binding proteins (LTBP) are believed to control the availability of TGF-beta in the extracellular milieu. To gain insight into the potential roles of LTBP in early development, we isolated the Xenopus LTBP-1 (xLTBP-1) cDNA. The cDNA encodes a protein similar to the mammalian LTBP-1 in both size and domain structure. In addition, we found a novel longer splice isoform of xLTBP. The RNAs for both forms of xLTBP displayed temporal regulation and the shorter transcript is expressed maternally. Both transcripts also display spatial regulation and are found in the dorsal mesoderm of the organizer. In animal cap experiments, LTBP-1 potentiates the activity of activin and nodal. The activity of LTBP-1 did not appear to require covalent association with activin as the addition of medium containing activin and LTBP-1 to animal caps enhanced the activin effect. These results indicate that LTBP-1 may be part of the regulatory system that establishes the threshold of morphogen activity for activins and nodals in the dorsal side of the embryo during gastrulation. (C) 2002 Elsevier Science (USA).