Virus-specific CD4+ and CD8+ cytotoxic T-cell responses and long-term T-cell memory in individuals vaccinated against polio
Virus-specific CD4+ and CD8+ cytotoxic T-cell responses and long-term T-cell memory in individuals vaccinated against polio
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DOI：
10.1128/jvi.79.10.5988-5995.2005
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发表时间：
2005-05-01
影响因子：
5.4
通讯作者：
Chow, M
中科院分区：
文献类型：
--
作者：
Wahid, R;Cannon, MJ;Chow, M
脊髓灰质炎病毒（PV）特异性CD 4（+）T细胞在接种脊髓灰质炎疫苗的个体中的存在已被证明，但CD 8（+）T细胞反应尚未被描述。在这里，我们在功能上表征了CD 4（+）T细胞应答，并首次表明树突状细胞和巨噬细胞可以在体外刺激疫苗接种者的PV特异性CD 8（+）T细胞应答。CD 4（+）T细胞和CD 8（+）T细胞均能分泌γ干扰素以应答PV抗原，并通过穿孔素/颗粒酶B介导的途径具有细胞毒性。此外，T细胞还识别并杀死Sabin 1疫苗感染的靶标。巨噬细胞刺激的CD 4（+）T和CD 8（+）T细胞最有可能代表在接种疫苗的个体中长期存在的记忆T细胞。因此，对PV疫苗接种的免疫不仅涉及有效的中和抗体滴度，还涉及长期的CD 4（+）和CD 8（+）细胞毒性T细胞应答。
The presence of poliovirus (PV)-specific CD4(+) T cells in individuals vaccinated against polio has been shown, but CD8(+) T-cell responses have not been described. Here, we functionally characterize the CD4(+) T-cell response and show for the first time that dendritic cells and macrophages can stimulate PV-specific CD8(+) T-cell responses in vitro from vaccinees. Both CD4(+) T and CD8(+) T cells secrete gamma interferon in response to PV antigens and are cytotoxic via the perforin/granzyme B-mediated pathway. Furthermore, the T cells also recognize and kill Sabin 1 vaccine-infected targets. The macrophage-stimulated CD4(+) T and CD8(+) T cells most likely represent memory T cells that persist for long periods in vaccinated individuals. Thus, immunity to PV vaccination involves not only an effective neutralizing antibody titer but also long-term CD4(+) and CD8(+) cytotoxic T-cell responses.