Effect of reduced glutathione (GSH) supplementation to Tris-egg yolk extender on chilled semen variables of dogs
Effect of reduced glutathione (GSH) supplementation to Tris-egg yolk extender on chilled semen variables of dogs
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DOI：
10.1016/j.anireprosci.2018.09.013
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发表时间：
2018-11-01
影响因子：
2.2
通讯作者：
Goericke-Pesch, Sandra
中科院分区：
文献类型：
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作者：
Andersen, Anne Hoeg;Thinnesen, Mette;Goericke-Pesch, Sandra
冷冻狗精液寿命缩短的一个原因是氧化应激。抗氧化剂谷胱甘肽（GSH）可提高冷冻解冻犬精子的活力，但其对冷冻犬精液的影响尚未研究。进行了由两部分组成的实验：将富含精子的组分SRF分开，用含有0、5或10 mM GSH的Tris-蛋黄（TEY）稀释液稀释，并分别在4 ℃下储存10天（第1部分; n = 19）或4天（第2部分; n = 11）。对于研究的第1部分，在稀释后第0、1、2、4和10天评估活动、存活、形态异常精子的百分比（%）和顶体偏离%。对于精子活力%，同时移取各SRF（0/5/10 mM GSH）所有三份等分试样的样本，并以随机顺序（分析时间点，TPA）进行分析。在研究的第2部分中，在4天储存期间进行运动性分析，并在移液后立即分析样品（第2部分）。大多数研究参数受储存时间的影响。对于运动性变量，GSH对环形CM（ANOVA，第1部分：P= 0.05，第2部分：P < 0.0001）和局部运动性LM（ANOVA，第2部分：P= 0.004）有影响。此外，CM的时间和精子处理之间存在相互作用的趋势（第2部分：P= 0.077）。总之，在本研究中，添加GSH（5/10 mM）对冷冻犬精液样本中的精子活力没有总体积极影响，这些样本在储存4天和10天期间具有良好的质量。
One reason for reduced longevity of chilled dog semen is oxidative stress. The antioxidant glutathione (GSH) improves viability of frozen-thawed dog sperm, but its effect on chilled dog semen has not been investigated. An experiment consisting of two parts was performed: Sperm rich fractions, SRF, were split, diluted with a Tris-egg yolk (TEY) extender containing 0, 5 or 10 mM GSH and stored at 4 degrees C for 10 days (Part 1; n = 19) or 4 days (Part 2; n = 11), respectively. For Part 1 of the study, percentage (%) of motile, viable, morphologically abnormal spermatozoa and % acrosomal deviations were assessed on days 0, 1, 2, 4 and 10 after dilution. For % sperm motility, samples from all three aliquots of each SRF (0/5/10 mM GSH) were pipetted simultaneously and analysed in a randomised order (time point of analysis, TPA). In Part 2 of the study, motility analysis was performed during 4 days storage and samples were analysed immediately after pipetting (part 2). Most investigated parameters were affected by storage time. For motility variables, there was an effect of GSH identified for circular, CM (ANOVA, Part 1: P= 0.05, Part 2: P < 0.0001) and local motility, LM (ANOVA, Part 2: P= 0.004). Furthermore, there was a trend for an interaction between time and sperm treatment for CM (Part 2: P= 0.077).In conclusion, in the present study there was not an overall positive effect of GSH addition (5/10 mM) on sperm motility in chilled dog semen samples that were characterised to be of good quality during 4- and 10-days of storage.