Structure and expression of a cDNA encoding a putative neoxanthin cleavage enzyme (NCE), isolated from a wilt-related tomato (Lycopersicon esculentum Mill.) library
Structure and expression of a cDNA encoding a putative neoxanthin cleavage enzyme (NCE), isolated from a wilt-related tomato (Lycopersicon esculentum Mill.) library
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DOI：
10.1093/jxb/48.12.2111
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发表时间：
1997-12-01
影响因子：
6.9
通讯作者：
Taylor, I
中科院分区：
文献类型：
--
作者：
Burbidge, A;Grieve, T;Taylor, I
9-顺式叶黄素的氧化裂解可能代表脱落酸（阿坝）生物合成的关键调控步骤。转座子标记的玉米（Zea mays）突变体vp 14提供了设计能够在体外催化该反应的VP 14融合蛋白所需的原始DNA序列数据。将番茄cDNA和衍生的氨基酸序列与玉米和催化广泛相似的氧化裂解反应的其它酶的cDNA和衍生的氨基酸序列进行比较。番茄中的北方分析的结果表明，响应于水分胁迫，该vp 14同源物的mRNA水平显著增加。
Oxidative cleavage of a 9-cis xanthophyll probably represents the key regulatory step in abscisic acid (ABA) biosynthesis. A transposon tagged maize (Zea mays) mutant vp14, provided the original DNA sequence data needed to design a VP14 fusion protein capable of catalysing this reaction in vitro, A cDNA encoding a similar protein has now been isolated from a wilt-related tomato (Lycopersicon esculentum Mill.) library, The tomato cDNA and derived amino acid sequence have been compared to those of maize and of other enzymes catalysing broadly similar oxidative cleavage reactions, The results of Northern analysis in tomato indicated that mRNA levels of this vp14 homologue increased dramatically in response to water stress.