Effects of solvent evaporation rate on the properties of protein-loaded PLLA and PDLLA microspheres fabricated by emulsion-solvent evaporation process
Effects of solvent evaporation rate on the properties of protein-loaded PLLA and PDLLA microspheres fabricated by emulsion-solvent evaporation process
复制标题
DOI：
10.1080/02652040210140706
复制
发表时间：
2002-07-01
影响因子：
3.9
通讯作者：
Liu, YZ
中科院分区：
文献类型：
--
作者：
Chung, TW;Huang, YY;Liu, YZ
本文研究了固定温度下改变环境压力的溶剂去除速率对 W/O/W 乳液-溶剂蒸发工艺制备的载白蛋白 PLLA 和 PDLLA 微球的形貌、粒径、包封率和释放模式的影响。对于采用快速溶剂蒸发速率 (FRSE) 或正常溶剂蒸发速率 (NRSE) 工艺制备的 PLLA 微球，形态差异较小。相比之下，不同的工艺确实影响了PDLLA微球的形貌。使用 FRSE 工艺制造的 PDLLA 微球观察到大（表面）孔隙，而使用 NRSE 工艺制造的 PDLLA 微球则观察到光滑的表面。与 NRSE 工艺制备的微球相比，FRSE 工艺制备的 PLLA 和 PDLLA 微球的粒径更小，白蛋白封装效率更低。 NRSE工艺制备的PLLA微球的药物包封率高于PDLLA微球，但FRSE工艺的情况并非如此。对于用 NRSE 工艺制备的 PLLA 和 PDLLA 微球，观察到白蛋白的初始爆发释放，而对于用 FRSE 工艺制备的微球，观察到较小的爆发释放。两种不同工艺制备的PLLA微球在药物释放的后续阶段表现出差异，但PDLLA微球则不然。
This paper investigated the effects of the rate of solvent removal by varying the ambient pressure at a fixed temperature on the morphology, particle size, encapsulation efficiency and release pattern of albumin-loaded PLLA and PDLLA microspheres, prepared by the W/O/W emulsion-solvent evaporation process. For PLLA microspheres prepared either with a fast rate of solvent evaporation (FRSE) or a normal rate of solvent evaporation (NRSE) process, the difference in morphology was minor. In contrast, the different processes did affect the morphology of PDLLA microspheres. Large (surface) pores were observed for PDLLA microspheres fabricated with a FRSE process, while a smooth surface was seen in those with a NRSE process. With the FRSE process, both PLLA and PDLLA microspheres showed smaller particle sizes and lower albumin encapsulation efficiencies than those prepared in the NRSE process. PLLA microspheres prepared with the NRSE process had higher drug encapsulation efficiencies than PDLLA ones, but this was not the case for the FRSE process. An initial burst release of albumin was observed for both PLLA and PDLLA microspheres prepared with the NRSE process, while a lesser burst release was seen for those prepared with the FRSE process. In subsequent stages of drug release, PLLA microspheres prepared with the two different processes showed differences, but this was not the case for PDLLA ones.