Seroprevalence of fasciolosis in Hokkaido sika deer (Cervus nippon yesoensis) from Hokkaido Prefecture, Japan revealed by ELISA using recombinant cathepsin L1
Seroprevalence of fasciolosis in Hokkaido sika deer (Cervus nippon yesoensis) from Hokkaido Prefecture, Japan revealed by ELISA using recombinant cathepsin L1
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使用重组组织蛋白酶 L1 进行 ELISA 检测，日本北海道梅花鹿 (Cervus nipponyesoensis) 的片形吸虫病血清阳性率
DOI：
10.1016/j.parint.2020.102222
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发表时间：
2021
影响因子：
1.9
通讯作者：
Ichikawa-Seki Madoka
中科院分区：
文献类型：
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作者：
Sato Hironobu;Hiraya Hiroki;Sugiyama Takutoshi;Fukumoto Shinya;Matsuyama Ryota;Yanagawa Yojiro;Nakao Ryo;Irie Takao;Taira Kensuke;Yamazaki Akiko;Hagiwara Katsuro;Yoshida Ayako;Kamata Yoichi;Ichikawa-Seki Madoka
片形吸虫病是一种由片形吸虫属肝吸虫引起的人畜共患疾病，已在日本北海道县的北海道梅花鹿（Cervus nippon yesoensis）中报告;然而，动物中的实际血清阳性率尚未得到充分评价。本研究的目的是分析北海道西卡鹿的疾病血清阳性率。以重组组织蛋白酶L1（rCatL 1）为抗原，建立了间接酶联免疫吸附试验（ELISA）检测抗肝片吸虫抗体的方法。ELISA的敏感性和特异性分别为84.6%和100%。从北海道县的20个地点采集的1109只北海道梅花鹿的平均血清阳性率为43.9%。成熟的鹿比年轻的个体表现出更高的血清阳性率;然而，即使是年轻的动物也可能成为疾病的宿主。监测北海道西卡鹿种群的感染水平不仅对畜牧业很重要，而且对预防人类片形吸虫病也很重要。
Fasciolosis, a zoonotic disease caused by liver flukes of the genusFasciola, has been reported in Hokkaido (Yezo) sika deer (Cervus nippon yesoensis) in Hokkaido Prefecture, Japan; however, the actual seroprevalence in the animal has not been adequately evaluated. The objective of the present study was to analyze the seroprevalence of the disease among Hokkaido sika deer. Recombinant cathepsin L1 (rCatL1) was used as an antigen for an indirect enzyme-linked immunosorbent assay (ELISA) to detect antibodies againstFasciolaflukes. The sensitivity and specificity of the ELISA were 84.6% and 100%, respectively. The average seroprevalence in 1109 Hokkaido sika deer from 20 locations in Hokkaido Prefecture was 43.9%. Mature deer showed higher seroprevalence than younger individuals; however, even younger animals may act as a reservoir for the disease. Monitoring infection levels in the Hokkaido sika deer population is important not only for the livestock industry, but also for preventing human fasciolosis.