Nature of the collagenous protein in a tumor basement membrane.
Nature of the collagenous protein in a tumor basement membrane.
复制标题
肿瘤基底膜中胶原蛋白的性质。
DOI：
10.1111/j.1432-1033.1978.tb12139.x
复制
发表时间：
1978
期刊：
影响因子：
--
通讯作者：
H. Wiedemann
中科院分区：
文献类型：
--
作者：
R. Timpl;George R. Martin;Peter Bruckner;Georg Wick;H. Wiedemann
EHS肉瘤(以它的发现者Engelbreth-Holm和它的主要特征R.Sarm命名)含有基底膜的细胞外基质。用稀醋酸从甲状腺功能亢进小鼠肿瘤中提取胶原膜成分，并用层析的方法与其他蛋白质分离。这种胶原蛋白的成分与报道的其他基底膜胶原蛋白相似。肿瘤胶原蛋白的多肽链通过二硫键连接，还原后在CI链和I型胶原成分之间迁移。从它得到的圆二色谱与从其他胶原蛋白得到的相似。每1000个残基中含有约280个甘氨酸残基。在基底膜胶原的二硫键减少后，用胃酶或胰酶处理后，产生含有三分之一甘氨酸的抗性片段。在胃酶处理的情况下，这些片段形成了210 nm长的节段长间隔微晶，在胰酶处理的情况下，这些片段形成了350 nm长的微晶。这种带型与其他胶原蛋白不同。针对肿瘤胶原蛋白制备的抗体被发现定位于肿瘤基质和已知的小鼠组织中的基底膜。这些数据表明，这些基底膜可能含有一种类似于肿瘤蛋白的胶原蛋白。
The EHS sarcoma (named after its discoverer, Engelbreth-Holm, and its primary characterizer, R. Swarm) contains an extracellular matrix of basement membrane. The collagenous component of the membrane was extracted with dilute acetic acid from tumors grown in lathyritic mice and separated from other proteins by chromatographic methods. This collagen has a composition resembling that reported for other basement membrane collagens. The polypeptide chains of the tumor collagen are linked by disulfide bonds and migrate after reduction intermediate between the CI chains und components of type-I collagen. The circular dichroism spectra obtained from it resemble those obtained from other collagens. The intact protein contained about 280 residues of glycine per 1000 residues. After reduction of disulfide bonds in the basement membrane collagen, treatment with either pepsin or trypsin gave rise to resistant fragments containing one-third glycine. These fragments formed segment-long-spacing crystallites 210 nm long in the case of those treated with pepsin and 350 nm long in the case of those treated with trypsin. The band pattern differs from that found with other collagens. Antibodies prepared against the tumor collagen were found to localize to the tumor matrix and to known basement membranes occurring in mouse tissues. The data suggest that these basement membranes may contain a collagenous protein similar to the tumor protein.