IL-4 induces eotaxin: a possible mechanism of selective eosinophil recruitment in helminth infection and atopy.
IL-4 induces eotaxin: a possible mechanism of selective eosinophil recruitment in helminth infection and atopy.
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IL-4 诱导嗜酸性粒细胞趋化因子：蠕虫感染和特应性中选择性嗜酸性粒细胞募集的可能机制。
作者：
M. Mochizuki;J. Bartels;A. I. Mallet;E. Christophers;J. Schröder
一些寄生虫感染以及过敏性和特应性皮肤病的共同特征是 Th2 淋巴细胞的参与和嗜酸性粒细胞 (Eos) 的真皮出现。由于 Th2 淋巴细胞显然不释放 Eo 引诱剂，因此我们解决了 Th2 细胞因子 IL-4 是否诱导其在真皮成纤维细胞中产生的问题。因此，我们用 IL-4 刺激成纤维细胞。上清液的 HPLC 研究揭示了单一的 Eo 趋化蛋白，该蛋白被纯化至均质，在 SDS-PAGE 分析中给出单一的 13-kDa 带。肽图分析和随后的氨基酸测序揭示了一种Eo选择性趋化因子，它由趋化因子嗜酸细胞趋化因子的N末端截短和O-糖基化形式的混合物组成。在这些条件下，其他趋化因子如 RANTES、MCP-3、MCP-4 或 MIP-1α 未检测为 Eo 趋化因子。使用逆转录酶 PCR 技术，我们发现 IL-4 剂量和时间依赖性地诱导真皮成纤维细胞中嗜酸细胞趋化因子 mRNA。 IL-4和TNF-α刺激导致三种生化不同嗜酸细胞活化趋化因子形式的释放增加10至20倍，每种形式由N端截短和O-糖基化变体的混合物组成，具有相同的主链氨基酸序列但不同的比活性。我们的研究结果支持这样的假设：IL-4 介导的皮肤反应中出现的嗜酸性粒细胞募集至少部分可能是由于 Th2 细胞因子介导的真皮成纤维细胞中嗜酸性粒细胞趋化因子的诱导。
A common feature of some parasitic infections and allergic and atopic skin diseases is the involvement of Th2 lymphocytes and the dermal appearance of eosinophils (Eos). Because Th2 lymphocytes apparently do not release Eo attractants, we addressed the question of whether the Th2 cytokine IL-4 induces its production in dermal fibroblasts. We therefore stimulated fibroblasts with IL-4. HPLC investigation of supernatants revealed a single Eo chemotactic protein, which was purified to homogeneity giving a single 13-kDa band upon SDS-PAGE analyses. Peptide mapping with subsequent amino acid sequencing revealed an Eo-selective chemotaxin, which consists of a mixture of N-terminally truncated and O-glycosylated forms of the chemokine eotaxin. Other chemokines such as RANTES, MCP-3, MCP-4, or MIP-1alpha were not detected as Eo chemotaxins under these conditions. Using reverse transcriptase-PCR techniques, we found that IL-4 dose and time dependently induces eotaxin mRNA in dermal fibroblasts. Stimulation with IL-4 and TNF-alpha caused a 10- to 20-fold increase of the release of three biochemically different eotaxin forms, each consisting of a mixture of N-terminally truncated and O-glycosylated variants having the same backbone amino acid sequence but different specific activities. Our findings support the hypothesis that eosinophil recruitment seen in IL-4-mediated skin reactions, at least in part, may be due to Th2 cytokine-mediated induction of eotaxin in dermal fibroblasts.
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盘尾丝虫介导的角膜炎：IL-4 缺陷小鼠产生细胞因子。
影响因子：
2.1
作者：
Pearlman,E;Lass,JH;Bardenstein,DS;Diaconu,E;HazlettJr,FE;Albright,J;Higgins,AW;Kazura,JW
通讯作者：
Kazura,JW
DOI：
10.1164/ajrccm/144.6.1274
发表时间：
1991-12-01
期刊：
AMERICAN REVIEW OF RESPIRATORY DISEASE
影响因子：
--
作者：
SEDGWICK, JB;CALHOUN, WJ;BUSSE, WW
通讯作者：
BUSSE, WW
影响因子：
15.9
作者：
POSTLETHWAITE, AE;HOLNESS, MA;RAGHOW, R
通讯作者：
RAGHOW, R
关于嗜酸性粒细胞在皮肤病中作用的当前观点。
影响因子：
13.8
作者：
Leiferman,KM
通讯作者：
Leiferman,KM
嗜酸性粒细胞的免疫生物学。
DOI：
10.1056/nejm199104183241607
发表时间：
1991
期刊：
The New England journal of medicine
影响因子：
--
作者：
Weller,PF
通讯作者：
Weller,PF