Panmictic structure of Helicobacter pylori demonstrated by the comparative study of six genetic markers
Panmictic structure of Helicobacter pylori demonstrated by the comparative study of six genetic markers
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DOI：
10.1016/s0378-1097(98)00080-9
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发表时间：
1998-04-15
影响因子：
2.1
通讯作者：
Picard, B
中科院分区：
文献类型：
--
作者：
Salaün, L;Audibert, C;Picard, B
我们比较了几个遗传标记的分析所获得的菌株的分类，以证明幽门螺杆菌的panmictic结构，以前提出的多位点酶电泳的研究。使用了一系列39株菌株，包括来自法国两个地区胃炎或溃疡患者的37株临床分离株，参考菌株CIP 101260和悉尼菌株（SSI菌株）。通过使用HindIII和Hae III的核糖体DNA的限制性片段长度多态性分析（核糖体分型），通过使用Hae III和MboI的PCR-RFLP的ureA-ureB和flaA基因的多态性分析，对它们进行了研究。通过vacA基因分型以及通过PCR检测cagA基因和插入序列IS 605的存在或不存在。有一个高水平的遗传多态性的研究菌株，38个核糖体，38个限制性内切酶的ureA-ureB基因，19个限制性内切酶的flaA基因和5个组合的信号和中间区域序列的vacA基因。使用每个标记进行的对应和层次聚类的析因分析显示，菌株的不同分类不相关。这表明菌株之间存在大量的遗传重组，支持了H. pylori物种。(C)1998年，欧洲微生物学会联合会。出版社：Elsevier Science B. V.
We compared the classifications of strains obtained by analysis of several genetic markers to demonstrate the panmictic structure of Helicobacter pylori, previously suggested by the study of multilocus enzyme electrophoresis. A series of 39 strains, including 37 clinical isolates from patients with gastritis or ulcers from two regions of France, reference strain CIP 101260 and the Sydney strain (strain SSI), were used. They were studied by restriction fragment length polymorphism analysis of ribosomal DNA (ribotyping) using HindIII and HaeIII, by polymorphism analysis of the ureA-ureB and flaA genes by PCR-RFLP using HaeIII and MboI. by vacA genotyping and by the presence or absence of the cagA gene and of the insertion sequence IS605 detected by PCR. There was a high level of genetic polymorphism over the studied strains, with 38 ribotypes, 38 restriction profiles for the ureA-ureB gene, 19 restriction profiles for the flaA gene and five combinations of the signal and mid-region sequences of the vacA gene. Factorial analysis of correspondence and hierarchical clustering performed using each marker revealed that the different classifications of the strains were not correlated. This suggests there is much genetic recombination between strains and supports the hypothesis of a panmictic structure for the H. pylori species. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.