CAPILLARY ELECTROPHORETIC SEPARATION OF PROTEINS USING STABLE, HYDROPHILIC POLY(ACRYLOYLAMINOETHOXYETHANOL)-COATED COLUMNS
CAPILLARY ELECTROPHORETIC SEPARATION OF PROTEINS USING STABLE, HYDROPHILIC POLY(ACRYLOYLAMINOETHOXYETHANOL)-COATED COLUMNS
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DOI：
10.1016/0021-9673(95)00413-4
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发表时间：
1995-11-24
影响因子：
4.1
通讯作者：
PESEK, JJ
中科院分区：
文献类型：
--
作者：
CHIARI, M;NESI, M;PESEK, JJ
采用聚丙烯酰胺乙氧基乙醇包被毛细管，实现了高效、高分辨率的毛细管电泳分离。该聚合物共价附着在先前用α -甲基丙烯氧基丙基官能团修饰的二氧化硅表面上。后者是通过在sih改性的熔融硅毛细管上催化甲基丙烯酸烯丙酯硅氢化反应实现的。在pH 8.5下使用的新型涂料的使用寿命是传统聚丙烯酰胺的两倍以上。
Capillary electrophoretic separations of high efficiency and resolution were obtained using poly(acryloylaminoethoxyethanol)-coated capillaries. The polymer was covalently attached to a silica surface previously modified with a alpha-methacryloxylpropyl functionality. The latter was realized by catalytic hydrosilylation of allyl methacrylate on an SiH-modified fused-silica capillary. The lifetime of the new type of coating used at pH 8.5 was more than twice that of conventional polyacrylamide.