Aquaporin 5 promotes tumor migration and angiogenesis in non-small cell lung cancer cell line H1299
Aquaporin 5 promotes tumor migration and angiogenesis in non-small cell lung cancer cell line H1299
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DOI：
10.3892/ol.2020.11251
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发表时间：
2020-03-01
期刊：
影响因子：
2.9
通讯作者：
Li, Xia
中科院分区：
文献类型：
--
作者：
Elkhider, Abdalkhalig;Wang, Bing;Li, Xia
非小细胞肺癌（NSCLC）占所有肺癌病例的大多数。水通道蛋白5（AQP 5）可能通过促进肺癌的发生和发展而参与非小细胞肺癌的发生和发展。本研究旨在利用NSCLC细胞和HUVECs确定AQP 5在迁移和血管生成中的作用。在非小细胞肺癌细胞系H1299中筛选AQP 1、3、4、5、8和9，目前的结果显示，与其他AQP基因相比，AQP 5 mRNA表达上调。在蛋白水平上，AQP 5在H1299细胞中的表达明显高于16 HBE细胞。与未转染细胞相比，H1299细胞中AQP 5敲低显著降低细胞迁移，如Transwell和伤口闭合试验所示。本研究进一步研究了H1299促进HUVEC血管形成的能力。将转染和未转染的H1299细胞的上清液用作HUVEC的条件培养基，并测量管形成。与未转染的细胞相比，AQP 5下调的细胞的上清液表现出显着低的管形成潜力。同样，与靶向AQP 5的小干扰RNA转染的细胞相比，对照细胞（si-NC）中的血管内皮生长因子显著增加。本研究发现，AQP 5下调可显著降低表皮生长因子受体的磷酸化水平和ERK 1/2通路的活性。总之，本研究表明，AQP 5在体外影响NSCLC的迁移和血管生成，并可能在体内表现出类似的作用。
Non-small cell lung cancer (NSCLC) constitutes the majority of all lung-cancer cases. Aquaporin 5 (AQP5) may be involved in NSCLC by promoting lung-cancer initiation and progression. The present study aimed to determine the role of AQP5 in migration and angiogenesis using NSCLC cells and HUVECs. AQPs 1, 3, 4, 5, 8 and 9 were screened in the NSCLC cell line H1299, and the present results showed that AQP5 mRNA was upregulated compared with the other AQP genes. At the protein level, AQP5 was significantly increased in H1299 cells compared with 16HBE cells. AQP5 knockdown in H1299 cells significantly decreased cell migration compared with untransfected cells, as demonstrated by both Transwell and wound closure assays. The present study further investigated H1299 ability to promote HUVEC vascularisation. The supernatants of both transfected and untransfected H1299 cells were used as conditioned medium for HUVECs, and tube formation was measured. The supernatant of AQP5-downregulated cells exhibited significantly low tube formation potential compared with untransfected cells. Similarly, vascular endothelial growth factor was significantly increased in control cells (si-NC) compared with cells transfected with small interfering RNA targeting AQP5. The present study found that AQP5 downregulation significantly decreased the phosphorylation level of epidermal growth factor receptor and the activity of the ERK1/2 pathway. In summary, the present study suggested that AQP5 influenced migration and angiogenesis in NSCLCs in vitro and may potentially exhibit similar in vivo effects.