RNA Extracted from Formalin-Fixed, Paraffin-Embedded Renal Biopsy Biospecimens: An Evaluation of Alternative Extraction Kits and the Effects of Storage Time
RNA Extracted from Formalin-Fixed, Paraffin-Embedded Renal Biopsy Biospecimens: An Evaluation of Alternative Extraction Kits and the Effects of Storage Time
复制标题
从福尔马林固定、石蜡包埋的肾活检生物样本中提取 RNA：替代提取试剂盒的评估和储存时间的影响
DOI：
10.1089/bio.2017.0013
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发表时间：
2017
影响因子：
1.6
通讯作者：
Zheng Chunxia
中科院分区：
文献类型：
--
作者：
Lu Yinghui;Zhang Mingchao;Jiang Qi;Yin Ru;Zhu Ping;Wang Hongtian;Liu Zhihong;Zheng Chunxia
世界各地的病理档案中保存了大量的福尔马林固定石蜡包埋（FFPE）肾活检组织。存档的 FFPE 样本是肾脏疾病分子研究的宝贵资源。 1, 2 使用现代基因组学和转录组学方法处理从 FFPE 组织中提取的核酸的能力将使大量常规储存的组织档案可用于转化研究。近年来，技术进步，例如灵敏的实时聚合酶链式反应 (RT-PCR) 系统的开发，允许快速、特异性地定量少量 mRNA。 3, 4 在本研究中，我们比较了在两个不同储存时间点（5 年和 10 年）从肾活检 FFPE 组织中提取 RNA 的两种试剂盒。
Abundant formalin-fixed and paraffin-embedded (FFPE) renal biopsy tissues have been preserved in pathology archives around the world. Archived FFPE samples represent an invaluable resource for molecular studies of renal diseases. 1, 2 The ability to use modern genomic and transcriptomic methods with nucleic acids extracted from FFPE tissues would allow enormous archives of routinely stored tissues to be used for translational research. In recent years, technological progress, such as the development of sensitive real-time polymerase chain reaction (RT-PCR) systems, permits rapid and specific quantification of small amounts of mRNA. 3, 4 In this study, we compared two kits for extracting RNA from renal biopsy FFPE tissues at two different storage time points: 5 and 10 years.