Palmitoleic Acid has Stronger Anti-Inflammatory Potential in Human Endothelial Cells Compared to Oleic and Palmitic Acids
Palmitoleic Acid has Stronger Anti-Inflammatory Potential in Human Endothelial Cells Compared to Oleic and Palmitic Acids
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DOI：
10.1002/mnfr.201800322
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发表时间：
2018-10-01
影响因子：
5.2
通讯作者：
Calder, Philip C.
中科院分区：
文献类型：
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作者：
de Souza, Camila Oliveira;Valenzuela, Carina A.;Calder, Philip C.
范围：脂肪酸 (FA) 可能影响内皮细胞 (EC) 功能，影响动脉粥样硬化形成和炎症过程。棕榈油酸 (POA) 被描述为一种抗炎 FA。然而，其对 EC 的影响尚未得到充分研究。本研究比较了 POA 与棕榈酸 (PA) 和油酸 (OA) 对 EC 炎症反应的影响。方法和结果：EAHy926 细胞（EC 谱系）暴露于 PA、OA 或 POA，并用肿瘤坏死因子 (TNF)-α 刺激。与 FA 自身的掺入相关，PA 导致花生四烯酸增加两倍，而 POA 则增加顺式异油烯酸的量。 PA（而非 OA）可响应 TNF-α 增强 IL-6 和 IL-8 的产生。相反，与 PA 相比，POA 减少单核细胞趋化蛋白 (MCP)-1、IL-6 和 IL-8 的产生。 TNF-α 增加了表面细胞间粘附分子 1 的表达，而此前 POA 则降低了表面细胞间粘附分子 1 的表达。 TNF-α 刺激会增加 NF kappa B、环氧合酶 (COX)-2、MCP-1 和 IL-6 基因的表达，并减少过氧化物酶体增殖物激活受体 (PPAR)-α 基因的表达。 PA 增强 MCP-1、IL-6 和 COX-2 基因的表达，而 POA 下调这些基因，降低 NF kappa B 的表达，并上调 PPAR-α 基因的表达。结论：POA 对 TNF-α 刺激的 EC 具有抗炎作用，并可能对抗内皮功能障碍。
Scope: Fatty acids (FAs) may affect endothelial cell (EC) function, influencing atherogenesis and inflammatory processes. Palmitoleic acid (POA) has been described as an anti-inflammatory FA. However, its effects on ECs are underexplored. This study compares the effects of POA with those of palmitic acid (PA) and oleic acid (OA) on EC inflammatory responses.Methods and Results: EAHy926 cells (EC lineage) are exposed to PA, OA, or POA, and stimulated with tumor necrosis factor (TNF)-alpha. Associated with the FA's own incorporation, PA induces a twofold increase in arachidonic acid, while POA increases the amount of cis-vaccenic acid. PA, but not OA, enhances the production of IL-6 and IL-8 in response to TNF-alpha. In contrast, POA decreases production of monocyte chemotactic protein (MCP)-1, IL-6, and IL-8 compared to PA. TNF-alpha increases surface intercellular adhesion molecule-1 expression previously decreased by POA. TNF-alpha stimulation increases the expression of NF kappa B, cyclooxygenase (COX)-2, MCP-1, and IL-6 genes and reduces the expression of peroxisome proliferator-activated receptor (PPAR)-alpha gene. PA enhances the expression of MCP-1, IL-6, and COX-2 genes, while POA downregulates these genes, decreases expression of NF kappa B, and upregulates PPAR-alpha gene expression.Conclusion: POA has anti-inflammatory effects on ECs stimulated with TNF-alpha and may counter endothelial dysfunction.